Studies on the effects of certain nutritional and environmental factors on the production of amylase by Bacillus subtilis BS5..

Author(s): Femi-Ola Titilayo Olufunke*, Olowe B. M.

Abstract

The effect of different starch and nitrogen sources on the production of amylase by the highly amylolytic isolates Bacillus subtilis BS5 from the gut of Amitermes evuncifer Silvestri was studied. Cocoyam starch proved to be the best carbon source for the enzyme production. Highest yield of amylase was achieved at pH 6.0. Enzyme synthesis occurred at 20 to 55oC with an optimum at 50oC. Amylase production was stimulated by Ca2+, Na+ and Mg2+ but inhibited by EDTA and HgCl2.

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