OXIDATIVE STRESS PARAMETERS AND ANTIOXIDANT STATUS IN MIDDLE AGED AMD ELDERLY SUBJECTS: AN AGE-RELATED COMPARATIVE STUDY

Author(s): Sayari Banerjee* and Swati Bhattacharyya

Abstract

Several studies in the Western countries have demonstrated that oxidative stress accelerates aging, enhances incidence of oxidative diseases and there is a slow decline of antioxidant status in older subjects but there is lack of significant works in this field in developing countries like India. The objective of the present study was to compare age-related oxidative stress vis-à-vis antioxidant defence in middle aged and older subjects. Measurement of concentration of protein carbonylation which is an early determinant of oxidative stress and end product of lipid peroxidation Thiobarbituric acid reactive substances helps to identify the effect of age related oxidative stress in human. Quantification of SOD in plasma and serum alphatocopherol was done to estimate the antioxidant status of the study population. The present hospital based non-interventional cross sectional study was designed to evaluate age- related oxidative stress as well as its impact in 50 middle aged (35-55y ) and 50 older subjects (>60y). On the basis of results obtained from this study it was evident that concentration of serum Thiobarbituric acid reactive substances and serum protein carbonylation were significantly higher in older subjects. It has been observed that enzymatic antioxidant serum Superoxide dismutase and free radical scavenging alfa-tocopherol were significantly lower in elderly age group than middle aged group. In conclusion, our findings do suggest that elderly subjects of the study population appear to be exposed to considerable amount of oxidative stress. A comparison between middle aged and older subjects suggests a decline of antioxidant status in elderly subjects

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