Haematological responses of heteroclarias fed dietary levels of Microdesmis puberula leaf meal.

Author(s): Anyanwu D. C.*, Udedibie A. B.I., Osuigwe DI2, Offor JI

Abstract

The haematological responses of feeding 35% isonitrogenous dietary levels of 0%, 5%, 10%, 15% and 20% Microdesmis puberula leaf meal on Heteroclarias (Heterobranchus bidorsalis x Clarias gariepinus) post fingerlings were assessed. These were fed to the fingerlings, randomly assigned to 5 treatments: 0%-control(TCN), 5%(TM1), 10%(TM2), 15%(TM3) and 20% (TM4) MPLM in 3 replicates of 15 post fingerlings each using 15 plastic aquaria of 250 x 150cm dimension. The fish were fed at 5% body weight twice daily within the experimental period of 56 days. Samples of blood were collected from the treatments bi-weekly and analyzed to evaluate some of the haematological profile, and data subjected to a one way analysis of variance (ANOVA). There were significant (p>0.05) differences among the treatments, except for the packed cell volume. The haemoglobin value for TM4 was significantly (p>0.05) higher than all the other treatments. TCN (control) was different (p<0.05) from TM1 and TM3. Besides, there was no significant (p>0.05) difference between TM1 and TM3 and between TCN and TM2 (p>0.05). The red blood cell values for TCN and Tm4 respectively were significantly (p>0.05) higher than the other treatments. These were followed by TM2 and TM3, which were in the same vein significantly (p>0.05) different from TM1. The white blood cell of the fish of TM4 was significantly (p>0.05) higher than the other treatments. TM3 on the other hand was significantly (p<0.05) more than TM1 and TM2. There was, however, no significant difference between TM1 and TM2, and between TM3 and TCN (control) (P>0.05). TM1 had the highest MCV value of 100.47mm3. This was followed by 92.53mm3, 91.30mm3, and 83.02mm3 for TM3, TM2 and TM4, respectively, while TCN (79.20mm3) was the least. The MCH values of 23.80pg, 22.68pg and 23.86pg for TM1, TM2 and TM4, respectively were significantly (p>0.05) higher than those of TCN (20.82pg) and TM3 (21.25pg).The mean cell Haemoglobin concentration value of 28.73% for TM4 however was significantly (p>0.05) higher than the rest of the treatments. This was followed by TCN (25.02%) and TM2 (24.84%) which were significantly (p>0.05) higher than TM3 (22.94%). TM2 (23.70%) was similar to the values for TCN and TM2.

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