Evaluation of in vitro mutagenic and antimutagenic activity of vitae elixxir by Ames salmonella microsome assay..

Author(s): Kharat Sanjay S*, Narke Vasant E.

Abstract

The Ames Salmonella / microsome mutagenicity assay (Ames test) is a short-term bacterial reverse mutation test speci�?¯�?¬�?cally designed to detect a wide range of chemical substances that can produce genetic damage leading to gene mutations. Vitae Elixxir is a mixture of nine herbal extracts with Chlorophyllin and Allicin. All herbs from Vitae Elixxir are medicinally very important with variety of roles in the different disorders or diseases, when studied individually by researchers. In spite of clinical use of Vitae Elixxir, no information is available on its toxicity, mutagenicity, antimutagenicity or anticarcinogenic potential. In present study, mutagenic and antimutagenic potential of Vitae Elixxir, was evaluated using plate incorporation assay, preincubation assay and modulation assay. The positive controls such as 4-NQNO, 2-AF, Danthron and MMS were used with and without S9. Ames Salmonella histidine reversion assay was studied using standard strains of Salmonella typhimurium TA97a, TA98, TA100 and TA102 with and without S9. In plate incorporation assay, Vitae Elixxir was not mutagenic at and up to 500 mg/plate. It exhibits the anti-mutagenic potential at 500 to 1000mg/plate in preincubation test. Vitae Elixxir is an extremely potent inhibitor of the mutagenicity at 1000mg and 500mg/plate and significantly dose dependent anti-mutagenic activity in TA98 and TA100 with and without metabolic activation. Vitae Elixxir would be beneficial for prevention of malignancy.

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