Estimation of antibodies against Pasteurella multocida b:2 in serum employing ELISA: comparison of different blocking reagents

Author(s): Subhash Kharb

Abstract

Haemorrhagic Septicaemia (HS), an endemic bacterial disease, is caused by specific serotypes of Pasteurella multocida and is one of the major economic diseases of bovines in south-east Asia including India. Sero-monitoring of vaccinal antibodies and definitive diagnosis of the disease is of utmost importance to combat the economic losses and ELISA system has remained the test of preference due to its versatility. Skimmed milk powder (SMP) and bovine serum albumin (BSA) respectively in concentration of 2, 3, 4% and 0.5, 1.0% have been compared as blocking reagents in ELISA for detection of antibodies against P. multocida. SMP and BSA blocking produced almost equal sensitivity. Results of the ELISA indicate that being inexpensive and easily available, SMP is a suitable blocking reagent for detection of antibodies against P. multocida B:2 for herd screening as well as in laboratories.

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