Effects of dietary excess of vitamin e and selenium on some production traits, lipid peroxide state and amount/activity of the glutathione redox system in African catfish Clarias gariepinus (siluriformes, clariidae) fingerlings

Author(s): Houssein Elbaraasi*, Miklós Mézes, László Horváth, István Csengeri, Katalin Németh, László Wágner, Krisztián Balo

Abstract

Purpose of present study was to investigate the effect of excess dietary amount of vitamin E and selenium also short-term starvation on some production traits and parameters of the lipid peroxide state and glutathione redox system in African catfish fingerlings. Three purified diets were formulated: diet 1 (control) contained 114 mg.kg-1 tocopherol and 0.43 mg.kg-1 selenium, diet 2 contained high level of tocopherol (245mg kg-1) and control level of selenium (0.5 mg kg-1), while diet 3 contained high level of selenium (2.5 mg kg-1) and control level of tocopherol (170 mg kg-1). Fingerlings of C. gariepinus (3.16-3.93 g) were investigated in four groups, three consumed the above mentioned diets and the fourth served as unfed control. The experiment lasted 30 days except in the case of the unfed group which was terminated on the 15th day because of high mortality. The vitamin E / selenium treated groups showed significantly (P£0.001) lower final body weight and specific growth rate as compared to the control. Vitamin E and selenium content of the fish body in diet 2 group increased significantly (P£0.001) as compared to the control. Vitamin E content did not changed in the unfed group, but selenium content decreased significantly (P£0.001) during the 15-days starvation period. Reduced glutathione (GSH) content increased significantly (P£0.05) only in the high selenium group. Glutathione-disulphide (GSSG) content, and glutathione peroxidase activity did not changed neither during the period of investigation and nor as effect of treatments, but the value of GSH/GSSG ratio was significantly higher (P£0.05) in the high selenium as compared to the high vitamin E group. Lipid peroxide state of the body, as was measured by the malondialdehyde content, changed as effect of age and/or maturation but not as effect of the treatments.

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