Cyanide hydratase production using acclimatized strain of Streptomyces phaeoviridae and its characterization.

Author(s): Shete H. G.*, Kapdnis B. P.

Abstract

Cyanide and cyanide compounds are produced on the industrial scale to use in the metal extraction, electroplating, polymer, steel, carbonization, organic chemicals, pharmaceuticals and agricultural product industries. Cyanide is a respiratory inhibitor and it affects the living cell by binding with the enzyme cytochrome C oxidase. Cyanide released in the stream cause poisoning to animals and fishes in the water bodies. As cyanide is highly toxic, it must be detoxified before discharging into the sewers. Potential cyanide degrader Actinomycete was isolated and acclimatized in the minimal medium containing 1000 ppm cyanide. It was then identified as Streptomyces phaeoviridae by using International Streptomyces Project Standard Tests. Cyanide degradation by this organism was studied. The mechanism of cyanide degradation was found to be enzymatic and the detection of formamide in the culture broth confirmed the cyanide hydratase [E.C. 4.2.1.66] activity. Parameters for cyanide hydratase production using Streptomyces pheoviridea were optimized. The enzyme was extracted and optimum conditions for its activity with respect to pH, temperature and substrate concentration were determined. The kinetics studied revealed the Km value as 33 mM and Vmax 35 mM/ml/min.

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