CONFIRMATORY EVIDENCE OTHER THAN 1HNMR SPECTROSCOPIC TECHNIQUE FOR COMPLEX FORMATION OF DICLOFENAC SODIUM WITH GO-GHRITA

Author(s): Bawankar RD, Sakarkar DM and Dorle AK

Abstract

Present investigation showing confirmatory evidence for complex formation of Diclofenac sodium (Dic) with Go�?¢�?�?��?Ghrita (GG) by various sophisticated techniques like FT�?¢�?�?��?IR, In-vitro release study, DSC, X�?¢�?�?��?ray diffraction and SEM other than 1NMR (which is reported earlier). Diclofenac sodium binary mixture in (1:0.5), (1:1), (1:2), (1:3) w/w proportions were prepared by adding to molten Go�?¢�?�?��?Ghrita kept over a water bath at 65�?¢�?�?��?70oC with continuous stirring. Organoleptic and Physico�?¢�?�?��?chemical properties of GG (procured from Magan Sangrahalaya, Wardha) were as per the specifications given in Ayurvedic Pharmacopoeia (AP) and Indian Pharmacopoeia (IP). All proportions were subjected for FT�?¢�?�?��?IR and in-vitro release behaviour (SGF, PH 1.2 for 2 Hrs and SIF, PH 6.8 for 7 Hrs) and on basis of it, optimized (1:1) w/w proportions were further analyzed and characterized for Differential Scanning Calorimetry, X�?¢�?�?��?ray diffraction and Scanning Electron Microscopy. Carbonyl stretching of benzene acetic acid group to slight higher frequency 9.23 % cm�?¢�?�?��?1 (increase) and (–C=O) linked (�?¢�?�?��?OH) band stretching to slight lower frequency 0.57 cm�?¢�?�?��?1 (decrease) shown in FT�?¢�?�?��?IR spectra, supported by in-vitro release of Diclofenac sodium 99.13±0.31% of (1:1) w/w in sustenance form than remaining. Retention of peaks with lesser intensity in binary mixture of drug with GG, revealed crystalline as well as non�?¢�?�?��?crystalline (amorphous) nature in X�?¢�?�?��?RD, Slight lowering of 17.60C Tm and enthalpy change of 8.51 % (loss) in DSC, and entrapment of Diclofenac sodium crystals in Dic�?¢�?�?��?GG binary mixture shown in SEM photographs, reflecting possible evidence for the formation of inclusion type complex between Diclofenac sodium and GG.

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