Association of Tumor Necrosis Factor-alpha and Interleukin-6 Gene Polymorphisms with Preeclampsia

Madhavi Puppala; V. Lakshmi Kalpana; Anuradha A; M. Sushma; G. Sudhakar; Sunil Kumar Polipalli

doi:10.21746/ijbio.2016.02.001

Association of Tumor Necrosis Factor-alpha and Interleukin-6 Gene Polymorphisms with Preeclampsia

Author(s): Madhavi Puppala*, V. Lakshmi Kalpana, Anuradha A, M. Sushma, G. Sudhakar, Sunil Kumar Polipalli

Abstract

Preeclampsia is a pregnancy-specific syndrome that may be dangerous especially to the fetus. Different cytokines have been found to be elevated in women with preeclampsia and may have possible roles in the development of this disorder. Alleles of the interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) genes are associated with preeclampsia in several studies in different populations. The aim of the present study was to investigate the relationship between IL-6 (G174C) and TNF-α (G-308A) gene polymorphisms with preeclampsia in North Coastal Andhra Pradesh of India. The TNFA (-308 A/G) and IL6 (-674G/C) genotypes were determined in 100 preeclamptic women and 100 normal pregnant women as control group, using allele-specific oligonucleotides-polymerase chain reaction method. Data was analyzed using chi-square and Fisher’s exact tests. TNF-α (G-308A) G/G genotype showed a significantly higher frequency among the preeclamptic group than the control group (odds ratio, 0.4603, 95% confidence interval, (0.2521- 0.8405); P = .005). G/A genotype also showed higher frequency among the preeclamptic group compared to control group (odds ratio, 2.508, 95% confidence interval, ((1.341-4.689); P = .001). IL-6 (G174C) genotype significantly higher frequency among the preeclamptic group than the control group (odds ratio, 0.4603, 95% confidence interval, (0.2521- 0.8405); P = .005). The present study might suggest a role for TNF-α (G-308A) and -174 GC of IL-6 genotype in the development of preeclampsia; suggesting that they are of differing genetic predisposition/pathophysiology.

10.21746/ijbio.2016.02.001

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