APPLICATIONS OF RAMAN SPECTROSCOPY FOR CHEMICAL CHARACTERIZATION AND PROTEIN CONFORMATION OF AGARICUS BISPORUS (LANGE) IMBACH. (AGARICOMYCETIDAE) SPORES

Author(s): Lamrood PY, Ralegankar SD and Harpale VM

Abstract

A Raman spectra was obtained for a spore suspension of an edible mushroom Agaricus bisporus using iRaman 2013 with a variable laser power (max. upto 300mW), near infrared 785 nm diode laser and resolution time 3 cm-1. The spore sample was exposed for 140s with excitation laser power 30mW to obtain optimum peaks. The spectra revealed Raman frequencies range from 242 to 2690 cm-1. Raman spectroscopy shows a strong S-S stretching vibrational band in the region of 500-550 cm-1 suggesting that the g-g-g form is the most preferred conformation. The spectra also exhibited the presence of various compounds such as C=C aliphetic chain, S-S, C-S, C-O-C, Sulfonic Acid, C≡C, P-H, Sulfhydryl Group (S-H), phosphorus acid selenate, C-Br, C-F, Si-H and notably isocynate. In the biological and environmental research this kind of analytical capability is very useful for the chemical identification of fungi, such as, Agaricus bisporus.

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