Anticancer activity of crude extract of eucalyptus globulus, Tinospora cordifolia on mcf-7 cell line

Author(s): Sr. Prema Kumari*, Louis Jesudas

Abstract

Methanolic crude extracts of Eucalyptus globulus and Tinosopra Cordifolia grown in natural and industrial polluted conditions were investigated for their anticancer activity against MCF-7 breast cancer cell lines to study the pollution effect on Cytotoxicity. It was carried out by the XTT assay using serial dilutions. The Cytotoxicity of individual plants and also the combined extracts of plants grown in polluted and natural area were carried out separately to check out the differences. The MCF – 7 cell lines were procured from National Centre for Cell Science, Pune. At final concentration of 200ug/ml the results indicated the cytotoxicity of individual plant extracts grown in polluted area was more than that of plants grown in natural conditions. The combined action of both plants at 200ug/ml concentration showed better results than the individual extracts. The cell inhibition of combined extracts of polluted area 81.2% and natural area 84% having IC50 values82.400 µg/mL, 91.980 µg/mL respectively. As such the plants that grown in industrial polluted area showed the better activity against MCF – 7 cell lines, further it could be recommended to study the pure components and their anti-cancer affectivity for the substitution of synthetic drugs.

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