Ameliorative effect of Nauclia latifolia leaf extract on valproic acid -induced neurotoxicity in oxidative stress rats: Role of p53 and S100B.
Author(s): Lucky Legbosi Nwidu*, Yibala Ibor Oboma
Abstract
Nauclear latifolia leaf (NLL) present with arrays of centra nervous system or neuropharmacological effects linked to uncommon display of phytochemical contents. The aim of our study was to evaluate the ameliorative effects of NLL in valproic aid-induced neurotoxic effect in oxidative stressed rats. The extract was prepared by first defatting in n-hexane before extraction with methanol after 72 hour maceration. The extract was subjected to phytochemical screening using standard procedure. The NLL extract obtained was administered in three doses (50, 100, 200 mg/ kg) after pre-treatment of the experimental animals with high dose valproic acid (VPA) (500 mg/kg) orally daily by subchronic exposure for 30 days. At the end of the experiment the animals were sacrificed and the prefrontal cortex (PFC) of the cerebrum abstracted and homogenized in ice for biochemical assays. The toxicological assay evaluated the weights changes in animals daily; the biochemical assays estimated the levels of antioxidants and lipid peroxidation; histopathological features were examined and the role of S100B and p53 protein expressions were investigated using histochemistry. The results indicated that intoxication of rats with vPA, induces change in weight in the animals. The three doses of NLL significantly ameliorated VPA-induced neurotoxicity. NLL extract significant downregulate the lipid peroxidation levels of malondialdehyde (NLL group vs. diseases control group (P<0.001), upregulate the levels of reduced glutathione (P<0.05 �?¢�?�?��?�?? 0.01), glutathione peroxidase (P<0.001), superoxide dismutase (P<0.001), catalase (P<0.05�?¢�?�?��?�??0.01) and glutathione-s-transferase (P<0.05�?¢�?�?��?�??0.001) in the PFC. These study demonstrate the efficacy of NLL in preventing neuronal insults by diminishing the expression of S100B and p53 protein in the PFC. The results of this findings indicate NLL effectiveness in ameliorating subchronic brain damaged induced by VPA by optimization antioxidants parameters, suggesting possible therapeutic potentials in Alzheimer�?¢�?�?��?�?�s diseases.
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