A RP-HPLC method development and validation for the estimation of solifenacin in bulk and pharmaceutical dosage forms

Author(s): Saroj Kumar Raul*, BVV Ravi kumar, Ajaya Kumar Patnaik

Abstract

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Solifenacin in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL min -1 was employed on XTerra C18 (150 × 4.6 mm, packed with 5 µm) column at ambient temperature. The mobile phase consisted of Acetonitrile: phosphate buffer 50:50 (v/v) and the detection wavelengths were at 210 nm. Linearity was observed in concentration range of 20-70 μg/mL. The retention time for Solifenacin was 2.4 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Solifenacin in pharmaceutical dosage forms.

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