Cover Image

Bioassay screening of the ethanolic extract of Tithonia diversifolia leaves on selected microorganisms

Anthoney Swamy T*, Jackie Obey K, Miyogo Edwin, Lasiti T Timothy

Abstract


The study was conducted to analyze the antibacterial activity of Tithonia diversifolia leaves. The plant was extracted using Ethanol and water in the ratio 9:1. From the study  the plant Tithonia diversifolia was found to inhibit the growth of  Staphilococcus epidermidis (36.80mm), Enterobacter aerogenes (31.20mm), Streptococcus α-hemolytic (29.60mm), Bacillus cereus (26.2mm), Escherichia coli (10.40mm) and Streptococcus γ-hemolytic (0.000mm)The data collected and documented in this paper is a scientifically justification that the plant can be used to treat against various diseases caused by Staphilococcus epidermidis and  Enterobacter aerogenes. However, further studies need to be done to identify the mode of action of the active compounds in the plant.

Keywords


Tithonia diversifolia; Antibacterial; Medicinal herbs; Leaves; Ethanol; Aqua.

Full Text:

PDF

References


Adedapo AA, Shabi OO, Adedokun OA, Antihelminthic efficacy of the aqueous extract of Euphorbia hirta (Linn.) in Nigerian dogs. Vet. Arch., 2005, 75 (1): 39-47.

Anthoney ST., Ngule CM, Obey J, Preliminary phytochemical screening of methanolic-aqua extract of Acanthospermum austral leaves. International Journal of Bioassays, 2013, 2(11): 1434-1439.

Anthoney ST, Jackie O, Ngule, CM, In vitro control of selected pathogenic organisms by Vernoniaadoensis roots. International Journal of Pharmacy and Life Sciences, 2013, 4(8): 2855-2859.

Anthoney ST, Ngule CM, Obey J, In Vitro Antibacterial activity of Methanolic-aqua extract of TragiabrevipesLeaves. Int. J. of Pharm. Life Sci, 2014, 5 (2): 3289-3294.

Anthoney ST, Obey JK, Miyogo EO, Terer E, In vitro antibacterial activity of the aqua extract of Phytolaccadodecandraroots against laboratory strains of selected human pathogenic organisms. Int. J. Bioassays, 2015, 4 (5), 3903-3909.

Anthoney ST, Obey J. K, Ngule C. M.In vitro Antibacterial Activity of Methanolic-Aqua Extract of Plectranthusagentatus Leaves. World Journal of Pharmaceutical Research (WJPR), 2014, 3(1): 339-349.

Anthoney ST and Omwenga J. Analysis of Phytochemical Composition of White and Purple Sweet Potato (Ipomoea batatas [L] Lam) root. Indian Journal of Advances in Plant Research (IJAPR), 2014, Vol. 1(3), 19-22.

Anthoney ST, Obey JK, Miyogo EO and Terer E, In vitro Antibacterial Activity of the Aqua Extract of PhytolaccaDodecandra Roots against Laboratory Strains of Selected Human Pathogenic Organisms. Int. J. Bioassays, 2015, 4: (5), 3903-3909.

Bork PM, Schmitz MC, Weimann C, Kist M, and Heinrish M, Nahua Indian medicinal plants (Mexico): Inhibitory activity on NF-Kb as an anti-inflammatory model and anti-bacterial effects. Phytomedicine, 1996, 3: 263-269.

Calzada JG, and Cicco JF, Aislamiento de Tirotundina a partir de Tithoniadiversifolia(Hemsl) Gray. Rev. Lattinoamer, Quim, 1978, 9: 202-203.

Gurib-Fakim A, Sewraj MD, Gueho J And Dulloo E, Medicinal Plants of Rodrigues. International Journal of Pharmacology, 1996, 34: 12-14.

Hammer KA, Carson CF, Riley TV, “Antimicrobial activity of essential oils and other plant extracts”, Journal of appliedMicrobiology, 1999, 86: 985-990.

Hongsbhanich L, Suttajit M, Kamtorn N, And Ounarom K, Insecticidal effect of Tithonia diversifolia. Journal of Resources. Con. Thailand, 1999, 11:13-25.

Ilesanmi FF, Olawoye TI, “A preliminary comparative phytochemistry of metabolites of orange (Citrus sinensis) and guava (Psidiumguajava) mistletoes and their host plants”, Journal of Medicinal Plants Research, 2011, 5(3): 340-343.

Jackie K. Obey and Anthoney Swamy T, In vitro evaluation of antibacterial activity of InfusedCola nitida Seeds. International Journal of Current microbiology and applied sciences (IJCMAS), 2014, Volume 3(10): 11-22.

Jackie K. Obey and Anthoney Swamy T, In vitro Antibacterial Evaluation of Ethanolic Extract of Thunbergia Alata Leaves Extract against Selected Microorganisms. Int. J. Bioassays, 2015, Vol. 4 (10): 4418-4422.

Johns T., Faubert, G. M., Kokwaro, J. O., Mahunnah, RL, and Kimanani, EK, Antigiardial activity of gastrointestinal remedies of the LUO of East Africa. Journal of Ethnopharmacology, 1995, 46: 17-23.

Kamden L, Messi HM, Ndongo NA, Mbi C, Njikam AP and Elobo S, Ethnobotanical investigations carried out in Mouloundon (Eastern Provice) and Zoetele (Southern Province). Revised Science Technology (Health SCI SER), 1986, 3(4): 59-68.

Kareru PG, Keriko JM, Kenji GM, Thiong’o GT, Gachanja AN, Mukiira HN, Antimicrobial activities of skincare preparations from plant extracts. Afr. J. Trad. CAM, 2010, 7 (3): 214 – 218.

Linthoingambi W, and Mutum S Singh, Antimicrobial activities of different solvent extracts of Tithonia diversifolia (Hemsely) A. Gray. Asian Journal of Plant Science and Research, 2013, 3(5):50-54.

Madureira MC, Martins AP, Gomes M, Paiva J, Cunha AP, and Rosario V, Antimalarial activity of medicinal plants used in traditional medicine in S. Tome and Principe Island. Journal of Ethnophamacology, 2002, 81: 23-29.

Miura T, Nosaka K, Ishii H, and Ishiada T, Antidiabetic effect of Nitobegiku (Tithonia diversifolia) in KK-Ay diabetic mice. Biol. Pharm. Bull, 2005, 28(11): 2152-2154.

NCCLS. Performance standards for antimicrobial susceptibility testing. 12th Informational Supplements, 2002, 22 (1), M 100– 511.

Obafemi SA, Suliamon TO, Akinpelu DA, Olugbade TA, “Antimicrobial activity of extracts and a germacranolide type sesquiterpene lactone from Tithonia diversifolia leaf extracts” African Journal of Biotechnology, 2006, 5 (12): 1254-1258.

Obey JK, Anthoney ST, Antibacterial Activity of Methanolic extract of Cola nitda Seeds on Selected Pathogenic Organism. International Journal of Current microbiology and applied sciences (IJCMAS), 2014, 3 (8): 999 -1009.

Jackie K,, Anthoney Swamy T and Ngule CM, Preliminary phytochemical and in Vitro Control of Selected Pathogenic Organisms by Ethanolic Extract of Garcinia kola Seeds. Int. J. Curr. Microbiol. App.Sci, 2014 3(4): 183-196.

Ogunfolakan O, Kolawole OS, Olowe AO. In vitro antimicrobial activity of T diversifolia leaf extracts on bacterial isolated from wound infection from Nigria Hospital. Research Journal of Medical Sciences, 2010, 4: 305-308.

Sharififar F, Moshafi MH, Dehgan-Nudehe G, Ameri A, Alishahi F Pourhemati A, “Bioassay Screening of the Essential oil and various extracts from 4 spices medicinal plants”, Pakistan Journal of Pharmaceutical Sciences, 2009, 22 (3): 317- 322.

Victor B, Owuyele VB, Wuraola CO, Soladdoye OA and Olaleye SB, Studies on the anti-inflammatory and analgesic properties of Tithonia diversifolia leaf extract. Journal of Ethnopharmacology, 2004, 90(4): 317-321.




DOI: http://dx.doi.org/10.21746/ijbio.2016.02.006

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.