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Effect of different extraction methods on total phenolic content and antioxidant activities of Raphanus sativus

Jamuna K. S.*, Ramesh C. K., Riaz Mahmood, Pallavi M., Aditya Rao S.J.

Abstract


Extraction is the first and crucial step in preparation of plant formulations. The use of bioactive compounds needs the most appropriate and standard method to extract the active components from plant material. Thus standardization of the extraction process is an important step for the establishment of a consistent biological activity, a consistent chemical profile or for quality assurance in production and manufacturing of herbal drugs or any herbal formulations. Solvent extractions are the most commonly used procedures to prepare extracts from plant materials due to their ease of use, efficiency and wide applicability. In the present study, different extraction methods are employed in order to assess the effect on total phenolic content and antioxidant activities in Raphanus sativus. Among the extraction methods applied, fresh material cold extraction was found to be superior followed by soxhlet and dried material cold extraction in terms of antioxidant activities tested. The results were discussed.

Keywords


Antioxidant; Extraction; In Vitro Scavenging Activities; Phenolics; Raphanus Sativus;

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References


Anonymous. South-East Asian Regional Workshop on Extraction Technologies for Medicinal and Aromatic Plants.organized by ICS-UNIDO in collaboration with the Central Institute of Medicinal and Aromatic Plants (CIMAP). 29 November-1 December, Lucknow, India. 2006.

Chandler S.F., Dodds J.H., The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Reports 2 (1993):1005-110.

Cohen J.H., Kristal A.R., Stanford J.L. Fruit and vegetable intakes and prostate cancer risk. J Natl Cancer Inst 92 (2000): 61–8.

Cover C.M., Hsieh S.J., Tran S.H., Hallden G., Kim G.S., Bjeldanes L.F. Indole-3-carbinol inhibits the expression of cyclin-dependent kinase-6 and 5. induces a G1 cell cycle arrest of human breast cancer cells independent of estrogen receptor signaling. J Biol Chem 273 (1998): 3838–47

Dinis T.C.P., Madeira V.M.C., Almeidam L.M., Action of phenolic derivates (acetoaminophen, salycilate, and 5- aminosalycilate) as inhibitors of membrane lipid peroxidation and peroxyl radicals scavengers. Ach Biochem and Biophy 315 (1994): 161-169.

Dobias P., Pavlikova P., Adam M., Eisner A., Benova B., Venture K. Cent Eur J Chem 8.1 (2010,):87-95.

Exarchou V., Nenadis N., Tsimidou M., Gerothanassis I.P., Troganis A., Boskou D. Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage and summer savory. J Agric Food Chem 50(2002):5294-5299.

Garrat D.C. The quantitative analysis of drugs Japan: Chapman and Hall. Phcog mag 3 (1964): 456-458.

Hsu B., Coupar I.M., Ng K. Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica, Food Chem, 98 (2006): 317-328.

Jamuna K.S., Ramesh C.K., Srinivasa T.R., Raghu K.L, in vitro antioxidant studies in some common fruits. International Journal of Pharmacy and Pharmaceutical Sciences 3.1 (2011) 60-63.

Khandelwal K.R. Practical Pharmacognosy techniques and experiments. 16th Edition. (Nirali Prakashan) 2006: 149-156.

Kothari V. Antimicrobial and Antioxidant Properties of Plant Products. Lambert Academic Publishing: Germany. 2011: 6.

Kothari V.O., Punjabi A.B., Gupta S.R. The Icfai Univ. J Life Sci 3.1 (2009): 55-60.

Oyaizu M, Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucose amine. Jap J Nutr, 44 (1986):307-315.

Prieto P., Pineda M., Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 269(1999): 337–341.

Ramesh C.K., Raghu K.L., Jamuna K.S., Govinden Soulange Joyce, Ranghoo Sanmukhiya Vijayanti Mala., Vijay Avin B.R. Comparative evaluation of antioxidant property in methanol extracts of some common vegetables of India. Annals of Biological Research-Scholars Research Library 2.2 (2011): 86-94.

Wattenberg L.W., Loub W.D., Inhibition of polycyclic aromatic hydrocarbon-induced neoplasia by naturally occurring indoles. Cancer Res 38 (1978)1410–3.

Wong S.P., Lai P.L., Jen H.W.K., Antioxidant activities of aqueous extracts of selected plants. Food Chem 99(2006): 775-783.

Yuan J.M., Gago-Dominguez M., Castelao J.E., Hankin J.H., Ross R.K., Yu M.C. Cruciferous vegetables in relation to renal cell carcinoma. Int J Cancer 77 (1998):211–6.




DOI: http://dx.doi.org/10.21746/ijbio.2015.12.0016

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