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Effect of different extraction methods on total phenolic content and antioxidant activities of Raphanus sativus

Jamuna K. S.*, Ramesh C. K., Riaz Mahmood, Pallavi M., Aditya Rao S.J.


Extraction is the first and crucial step in preparation of plant formulations. The use of bioactive compounds needs the most appropriate and standard method to extract the active components from plant material. Thus standardization of the extraction process is an important step for the establishment of a consistent biological activity, a consistent chemical profile or for quality assurance in production and manufacturing of herbal drugs or any herbal formulations. Solvent extractions are the most commonly used procedures to prepare extracts from plant materials due to their ease of use, efficiency and wide applicability. In the present study, different extraction methods are employed in order to assess the effect on total phenolic content and antioxidant activities in Raphanus sativus. Among the extraction methods applied, fresh material cold extraction was found to be superior followed by soxhlet and dried material cold extraction in terms of antioxidant activities tested. The results were discussed.


Antioxidant; Extraction; In Vitro Scavenging Activities; Phenolics; Raphanus Sativus;

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