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Fungal biomass production using Deproteinised Leaf Juice (DPJ) of Trigonella foenum-graecum

Sayyed I. U.*

Abstract


Isolation of leaf protein from the green foliage which releases Deproteinised Leaf Juice (DPJ). DPJ contains sugars, amino acids, minerals etc. Studies were conducted to investigate the possibilities to grow fungi on the deproteinised juice prepared from Trigonella foenum- graecum L. for the production of fungal biomass. Fresh green foliage of spinach was harvested early in the morning at a preflowering stage. It was pulped using pulper and subsequently pressed and the juice released due to the pressing was collected. The juice released during fractionation was employed for the preparation of LPC by head coagulation and the DPJ released after precipitation and isolation of proteins in juice was collected. The samples of DPJ were dried in hot air oven at 650 C. The conventional GN medium was prepared. Simultaneously, the dry DPJ was dissolved in distilled water at various concentrations and used as a medium for growing fungi. The flasks were inoculated for 8 to 12 days after inoculation. The fungal biomass was harvested by filtration through Whatman filter paper. The mycelial biomass was dried along with the filter paper in an oven at 65±50C till constant weight. The yield of mycelial dry weight (MDW) was then recorded. During all experiments, the culture filtrate was collected after harvesting the biomass. During present investigation that for maximum production of mycellial biomass of fungi on deproteinized juice of Trigonella foenum- graecum L in comparison with GN media.


Keywords


Fungal biomass; fungal medium; DPJ; etc.

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References


Ajaykumar, K. and Mugikar, A.M. Mendel, 1990a. 7(3-4): 389.

Ajaykumar, K. and Mungikar, A.M. Geobios, 1990b. 17: 188.

Ajaykumar, K. and Mungikar, A.M. Mendel, 1990c. 7(3-4): 389.

Ansari, S. N., & Iliyas, S. A Comparative Study of Protein Structure Visualization Tools for Various Display Capabilities. Bioscience Discovery: An International Journal of Life Sciences, 2011. 2(2). P 222-226. 5p.

Balsundaram, C.S., Chandramani, R., Krishnamoorthy, K.K., and Balalrishnan, T.C. Madras Agric. J. 1975. 62: 230.

Basole, S.G. “Studies on silages made from forage crops”. Ph.D. Thesis, Dr. Babasaheb Ambedkar Marathwada Uiniversity, Aurangabad. 1994.

Batra, U.R., Deshmukh, M.G. and Joshi, R.N. Indian J. Plant Physiology. 1976. 19:211.

Baviskar, V., Gogle, D.P. and Mungikar, A.M. In “Frontiers of Botany”. 1999.

Brandy, C.J. Biochem. J. 1961.78: 631.

Bryant A. M., Carruthers, V. R. & T.E. N.Z. J. Agric. Res. 1983.26; 79.

Cheesman, G.C. Occ. Symp. Br. Grassld. Soc. 1977.9:39.

Connel, J. & Houseman, R.A. in “Green Crop Fractionation” (Wilkins, R.J., Ed.), Brit. Grassld. Soc, Occas. Symp. 1977.Pp.57.

Consten, Gordon, and Martin. Biochem. J., 1944. 38, 224.

Daavys, M.N.G., Pirie, N.W. and Street, G. Biotech. Bioengng. 1969. 11: 517.

Dakore, H.G. and Mungikar A.M. Pollution Rees. 1986.5: 123.

deFremery, D., Edwards, R.H., Miller, R.E., Knuckles, B.E., Bickoff, E.M. and Kohler, G.O. In Twelfth Technical Alfalfa Conference Proceedings, American Dehydrations Association, Kansas, 1973. pp.99.

Deshpande, K.S. and Joshi, R.N. Indian J. Microbiol. 1973.9(4): 101.

Dev, D.V. Batra, U.R. and Joshi, R.N. J. Sci. Fd. Agric. 1974.25: 725.

H.D., Ed.) AVI Publishing Co., Inc., Wesport, Connecticut, pp.9.

Iliyas, S., & Ansari, S. N. A Comparative Study of Different Properties Provided by Protein Structure Visualization Tools. International Journal of Basic and Applied Chemical Sciences, 2013. Vol. 3 (1), pp.1-6.

Iliyas, S., & Badar, K. V. LPC: A Good Source of Cyanocobalamine (B12), Ascorbic Acid (Vitamin C) and Folic Acid (Vitamin B9). Journal of Experimental Sciences, 1(2). 2010a Pp. 12-14.

Iliyas, S., & Badar, K. V. Estimation of Thiamine, Riboflavin and Pyridoxine from LPC of Some Plants. Journal of Experimental Sciences, 2010b. 1(2). Pages 15-17.

Jadhav R. K. & Mungikar A.M. Mitotic Inhibition & Chomosomal Aberrations induced by Deproteinised Leaf Juice of Lucerne (Medicago sativa Linn.) In root tips of onion (Alium sepa). Inp. J. Mendel. 1998. 15 (1&2); 21-22.

Jadhav R.K., Effect of DPJ from Eicchornia crassipes. Bioinfolet; 2009b.6 (3), 246-248.

Jadhav R.K., A.M. Mungikar, Effect of DPJ on growth of cowpea, Indian Journal of Aquatic Biology, 1998.13(1-2):144-145.

Jadhav R.K., A.M. Mungikar, Preparation of LPC, Geobios. 2001;32;101-102.

Jadhav R.K., Phytotoxic effect of DPJ of lucerne on pea. Bioinfolet; 2009a.6(3), 240-241.

Jones, A.S. & Houseman, R.A. Rep. Rowett Rees Inst. 1975,31; 136.

Josephin, E. D. B., & Sayyed, I. U. Deproteinised Leaf juice as a medium for fungal growth and for production of Protease. Geobios, 2005. 32(4), 282.

Joshi, R.N. & Mungikar, A.M. Proc. Indian Acad. Sci. (Plant Sci.) 1983.92; 35.

Joshi, R.N. 1978. Proc. II Int. Green Crop Drying Conf. University of Saskatchewan.

Joshi, R.N., Savangikar, V.A., and Patunkar, B.W. Indian Bot. Reporter. 1984.3: 136.

Macpherson, H.T. J. Sci. Fd. Agric. 1952.3: 362

Mainderkar, K.G. “Studies on the leaf extracts obtained during fraction of green vegetation”, Ph.D. Thesis, Marathwada University, Aurangabad. 1988.

Morries, T.R. In “Green crop fractionation” (Wilkins, R. J., Ed.). Beit. Grassld. Soc. Occas. Symp. 1977.p. 9.

Mungikar, A.M. Indian bot. Reporter. 1982.1: 107.

Mungikar, A.M. Final Report of U.G.C. minor research project entitled “The use of sugarcane crusher forgreen crop fractionation and leaf protein production”. Dr. Babasaheb Ambedkar Marathwada1998.

Mungikar, A.M. Geobios. 2002.29: 183-184.

Mungikar, A.M., Batra, U.R., Tekale, N.S. and Joshi, R.N. Expt. Agric. 1976. 12: 353.

Mungikar, A.M., Prasad, V.L., Joshi, A.L. Presented at the 21st Veterinary of leaf protein concentrate obtained from various green foliages”. E thesis. 1978.Pp 1-5.

Ostrowski Meisser, H.T. In “Leaf protein concentrates” (Telek, L. and Graham, 1983.

Panse, V.G. and Sukhatme, P.V. “Statistical methods for Agricultural workers”. I.C.A.R., New Delhi. 1978.

Pirie, N.W. Ed. “Leaf protein: its agronomy, preparation, quality and use” (Pirie, N.W. Ed.), IBP Handbook no.20, Blackwell Scientific Publications, Oxford and Edinburgh. 1971.

Pirie, N.W. “Leaf protein and other aspects of fodder fractionation”. Cambridge University Press, London. 1978.

Prasad, V.L., Dev, D.V., Patil, R.E., Joshi, A.L. and Rangnekar, D.V. Indian J. Dairy Sci., 1977a.30: 154.

Prasad, V.L., Patil, B.R., Joshi, A.L. and Rangnekar, D.V. Indian Dairyman, 1977b. 29: 669.

Rajesh K. Jadhav, Distribution of ash, calcium and phosphorus in the byproducts of green crop fractionation. International Journal of Bioassays. 2015.4.9 4329-4332.

Rajesh. K. Jadhav, Distribution of nitrogen content in GCF products, Bioinfolet, 2014. 11 (2C);722-725.

Raymond, W.F. and Harris, C.F. J. British Grassland Society, 1957.12: 166.

Ream, H.W., Jorgensen, N.A., Koagel, R.G. and Bruhn, H.D. In “Leaf protein concentrates” (Telek L. and Graham, H.D. Ed.) AVI Publishing Company. Inc., West Port, Connecticut, 1983. pp.467.

Ream, H.W., Smith, D. and Walgenbach, R.P. Agron. J. 1977.69:685.

Reddy, G.S. and Mungikar, A.M. Indian. J. Agric. Biochem, 1988. 1(2):27.

Relwani, L.L. “Fodder Crop and grasses”, Indian council of Agricultural Research”, New Delhi. 1979.

Sadasivam, S. and Manickam A. “Biochemical Methods for Agriculture Sciences”, Wiley Eastern Ltd., New Delhi, 1992.pp.122-124

Sami Patel and Sayyed I.U. A review on implementation of image processing in Agriculture sector. Sci. Res. Rept., 2014. 4(1):71-78.

Sami Patel and Sayyed I.U. Impact of information technology in agriculture sector, International Journal of Food, Agriculture and Veterinary Science, 2014.Vol. 4 (2) May-August, pp. 17-22.

Sayyed I.U. Study of LPC and PCR Prepared from Radish (Raphanus sativus Linn.) Plant Sciences Feed, 2011. 1(6): 88-92.

Sayyed I.U. Effect of additives on the yield of LPC prepared from Medicago Sativa Linn., Indian Journal of Plant Sciences, 2014c. 3 (2) April -June, pp.10-12.

Sayyed I.U. Effect of different flours on α- amylase production, Am. J. Sci. Ind. Res, 2014b. 5(3): 104-109.

Sayyed Iliyas and Farhana S. Sarkhawas. A comparative study of MSA tools based on sequence Alignment feachers and platform independency to select the appropriate tool desired, Proceedings of National Conference on Data Mining, 2011.PP: 82-86, http://www.excelpublish.com.

Sayyed Iliyas. Production of amylase of DPJ of four different plants, International Journal of Basic and Applied Chemical Sciences, 2013.Vol. 3 (4), pp.48-61.

Sayyed, I. U. Effect of additives on chlorophyll content in wet LPC prepared from juice of Medicago sativa Linn. Bioinfolet-A Quarterly Journal of Life Sciences, 2010.7(3), 251-259.

Sayyed, I. U., & Mungikar, A. M. Changes in chlorophyll content of Lucerne leaf juice during storage. Geobios. 2003. 30(4), 299-300.

Sayyed, I. U., & Mungikar, A. M. Use of Deproteinised Leaf Juice (DPJ) in Microbial Biotechnology. Pollution Research, 2005.24(2), 459.

Sayyed, I.U. Production of α-amylase on deproteinised leaf juice prepared from different plants. Plant Sciences Feed, 2014a; 4 (4): 31-35.

Shah, F.H. In “Leaf protein concentrates” (Telek L. and Graham, H.D. Ed.) AVI Publishing Company. Inc., West Port, Connecticut, 1983. pp.760.

Shahane, J.M. “Studies on physiological aspects of forage fractionation” Ph.D. Thesis, Marathwada University, Aurangabad. 1986.

Shaikh, A., and Sayyed, I.U. The impact of mathematical tools on cellular and molecular Biology. International Journal of Bioassays, 2014.3(05), 3011-3015.

Singh, N. J.Sci. Fd Agric., 1962.13.

Tracey, M.V. Biochem. 1948.J.42: 281

Wilkins, R. J., Health, S. B., Roberts, W. P. and Foxell, P. R. In “Green Crop Fractionation” 1977. (Wilkins, R.J, Ed), Br. Grassld, Soc Occas. Symp. 9, Hurley.

Yoshida, S., Forno, D.A., Cock, J.H. and Comez, K.A. Laboratory Manual for Physiological studies of rice. The International Rice Research Institute, Philippines, 1976. pp.43.




DOI: http://dx.doi.org/10.21746/ijbio.2015.12.0014

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