Cover Image

Development of RP-HPLC method for simultaneous estimation of Aspirin, Ramipril, Atenolol and Atorvastatin

N. Sanni Babu*, S. Mutta Reddy


Polypill is envisaged to be a combination anti CVDs contained in the same dosage form unit.  In that a polypill or Multicomponent cardiovascular pill (MCCP) is closest to ‘Fixed dose combinations (FDC)’ or its synonym ‘Fixed dose Combination-Finished pharmaceutical product’ (FDC-FPP). Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A simple, rapid, precise, and reliable reverse phase HPLC method was developed for the separation and estimation of four drugs Aspirin, Ramipril, Atenolol and Atorvastatin in cardiovascular polypill based synthetic mixture. The estimation was carried out using Inertsil ODS-3V (250 mm × 4.6 mm, 5 𝜇m) column; mobile phase consisting of acetonitrile, methanol and buffer (pH=2.5); flow rate of 0.8 mL/min and ultraviolet detection at 225 nm. All the drugs were properly eluted within run time of 10 min with retention times about 4.1 min for Aspirin; 5.9 min for Atorvastatin; 7.2 min for Atenolol; and 8.3 min for Ramipril 8.333 min, respectively. The method was validated as a final verification of method development with respect to precision, linearity, accuracy, ruggedness, and robustness. This validated method was successfully applied to the commercially available pharmaceutical dosage form, yielding very good and reproducible result.


Simultaneous Estimation; Aspirin; Ramipril; Atenolol and Atorvastatin

Full Text:



International Conference on Harmonization, ICH Harmonized Tripartite Guidelines- Validation of analytical procedures: methodology, Fed. Regist. 1997.

Krull IS, Swartz M. Analytical Method Development and Validation for the Academic Researcher: Validation View Point. Analytical Letters. 1999; 32, 1067- 1080.

Craig S. Young and Raymond. J. Weigand. An efficient approach to column selection in HPLC Method Development, www.alltech

W. Grimm, in J.T. Cartensen and C.T. Rhodes (Eds) Drug Stability, Principles and Practices, Marcel Dekker, New York, 2000.

Vijay Kumar, Bhagwat Prasad, Saranjit Singh. Pharmaceutical issues in the development of a polypill for the treatment of cardiovascular diseases. Drug Discovery Today: Therapeutic Strategies. 2008; 5(1):63-71.

Elizabeth G. Nabel. Cardiovascular disease. N Engl J Med. 2003 Jul 3; 349:60-72.

Neela M. Bhatia, Sachin B. Gurav, Swapnil D. Jadhav & Manish S. Bhatia, RP-HPLC method for simultaneous estimation of Atorvastatin calcium, losartan potassium, atenolol, and Aspirin from tablet dosage form and plasma, Journal of Liquid Chromatography & Related Technologies, Volume 35, Issue 3, 2012,428-443.

A.K.M. Pawar, K. Sreekanth, A.B.N. Nageswararao, d. Gowrisankar an Isocratic method for the simultaneous estimation of Aspirin, ramipril and simvastatin by RP-HPLC, International Journal of Pharmacy and Pharmaceutical Sciences, Vol 4, Suppl 1, 2012, 425-428.

R.S. Murthy, Maram Ravi Kumar, Useni Reddy Mallu, Hanimi Reddy Bapatu, A Simple RP-HPLC method for simultaneous analysis of Aspirin, Atenolol, Hydrochlorothiazide, Ramipril and Simvastatin in pharmaceutical solid dosage forms International Journal of Science Innovations and Discoveries, 2012, 2 (1), 137-151.

Savita S Yadav, Janhavi R Rao. RP-HPLC method for simultaneous estimation of Aspirin, Ramipril, Hydrochlorothiazide, Simvastatin and Atenolol from pharmaceutical dosage form, International Journal of Pharmacy and Pharmaceutical Sciences Vol 6, Issue 9, 2014, Vol 6, Issue 9, 443-448.

Satheesh K. Shetty, Koduru V. Surendranath, Pullapanthula Radhakrishnanand, Roshan M. Borkar, Prashant S. Devrukhakar, Johnson Jogul, Upendra M. Tripathi Quantitative Application to a Polypill by the Development of Stability Indicating LC Method for the Simultaneous estimation of Aspirin, Atorvastatin, Atenolol and Losartan Potassium, American Journal of Analytical Chemistry, 2010, 2, 59-69.



  • There are currently no refbacks.

Copyright (c) 2015 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.