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Is AST? A cardiogenic marker for fluoride induced toxicity in animal model

Mujahid M*; Ramaswamy C and Shobha M


The aim of the study was to investigate the effect of sodium fluoride on serum lipid profile and AST (aspartate aminotransferase) enzymes and the possible protective role of selenium against fluoride toxicity on serum AST levels and lipid profile. 18 male wistar albino rats were divided into 3 groups control, experimental and interventional groups. Both experimental and interventional group were given 200 ppm fluoride water, whereas the control group received RO filter for drinking. Interventional group received 5mg/kg body weight/day selenium as a single dose orally by using oral gavages for 20days at the end of experimental protocol. Chronic exposure of fluoride resulted in an increase in serum triglyceride and VLDL levels in both the experimental and interventional group. The levels of HDL decreased in experimental and interventional groups while no change was observed in serum cholesterol and LDL levels when compared to control group. There was an increase of serum AST levels in experimental and interventional group. Upon supplementation of selenium for 20 days, resulted in decreased triglyceride, VLDL levels, increased HDL levels and Decreased AST levels in interventional group when compared to experimental group which was statistically significant with control and experimental group. Reversals of these effects were probably due to selenium which is an antioxidant and ameliorative in nature. Supplementation of selenium in fluoride endemic zones can prevent cardiovascular diseases. Screen of AST levels along with lipid profile may probably help in diagnosis of cardiovascular diseases in people residing at fluoride endemic zones.


Fluoride; Selenium; AST, Cholesterol; Triglycerides; HDL; LDL and VLDL

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Sinha M, Manna P and Sil PC. Terminalia arjuna protects mouse hearts against sodium fluoride-induced oxidative stress. J Med Food.11.4 (2008): 733-740.

Nabavi SF, Nabavi SM, Mirzaei M and Moghaddam AH. Protective effect of quercetin against sodium fluoride induced oxidative stress in rat's heart. Food Funct. 3.4 (2012b): 437-441.

Nabavi SM, Nabavi SF, Eslami S and moghaddam AH. In vivo protective effects of quercetin against sodium fluoride-induced oxidative stress in the hepatic tissue. Food Chem. 132 (2012c): 931-935.

Czerny B, Put A, Mysliwiec Z and Juzyszyn. The influence of quarcentin on some parameters of lipid metabolism in rats chronically exposed to ammonium fluoride. Fluoride. 33 (2000):27-32.

Grucka-Mamezar E, MachoyZ, Tarnawski R, Birkner E, Mamcza A. Influence of long-term sodium fluoride administration on selected parameters of rat-blood serum and liver function. Fluoride. 30 (1997):157-164.

Bennis A, Kessabi M, Hamliri A, De La Farge F, Braun JP . Plasma biochemistry of adult goats with chronic fluoride poisoning in morocco. Fluoride. 26 (1993): 241-246.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72 (1997): 248-254.

Chtourou Y, fetoui H, Sefi M, Trabelsi K, Barkallh M, Boudawara T, Kallel H and Zeghal N. Silymarin, a natural antioxidant, protects cerebral cortex against manganese-induced neurotoxicity in adult rats. Biometals, 23.6 (2010): 985-996.

Ellman GL. Tissue sulphydryl group. Arch Biochem Biophys. 82 (1959): 70-77.

Lee DY and Liu Y. Molecular structure and stereochemistry of silybin A, silybin B, isosilybin A, and isosilybin B, Isolated from Silybum marianum (milk thistle). J Nat Prod. 66 (2003): 1171-1174.

Liu G, Chai C and Cui L. Fluoride causing abnormally elevated serum nitric oxide levels in chicks. Environ Toxicol Pharmacol. 13 (2003): 199-204.

Ranjan R, Swarup D and Patra RC. Oxidative stress indices in erythrocytes, liver, and kidneys of fluoride exposed rabbits. Fluoride. 2009, 42.2 (2009): 88-93.

Rao PR and Viswanath RK. Cardio protective activity of silymarin in ischemia-reperfusion-induced myocardial infarction in albino rats. Exp Clin Cardiol. 12.4 (2007). : 179-187

Sinha M, Manna P and Sil PC. Terminalia arjuna protects mouse hearts against sodium fluoride-induced oxidative stress. J Med Food. 11.4 (2008): 733-740.

Friedel R, Diederichs F, Lindena J. Release and extracellular turnover of cellular enzymes. In advanced clinical enzymology. (1979).

Eman Salah Abdel-Reheim, Hanan Abdel-Hameid Abdel-Hafeez, Basant Mohamed Mahmoud and Eman Nazeem Abd-Allah. Effect of Food Additives (Monosodium Glutamate and Sodium Nitrite) On Some Biochemical Parameters in Albino Rats. International Journal of Bioassays. 3.08 (2014): 3260-3273



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