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Evaluation of antidiabetic activity of an important medicinal plant Aloe cim-sheetal leaf extract on streptozotocin induced diabetic rats

Chandra Sekhar Singh B.*, Surekha M., Rama Rao B., Roja Rani A.

Abstract


Diabetes mellitus belongs to the group of metabolic diseases characterized by hyperglycemia and defective protein metabolism. This study was undertaken to study the antidiabetic effect of the methanol extract of Aloe CIM- Sheetal (CAL 14) on streptozotocin induced diabetic rats. Diabetes was induced experimentally in fasted SD rats (170-180gm) by intra-peritoneal injection of STZ at a dose of 55 mg/kg b.w. and leaf extract of Aloe: control rats received (0.4 ml of 0.1 M of cold citrate buffer). Diabetic rats treated with Glibenclamide (0.60mg /kg) decreased blood glucose level from 298.66 ± 2.16 mg/dl to 192.00 ± 4.14 mg/dl (58.94%). Diabetic rats treated with Aloe 300 mg/kg showed decreased blood glucose levels from 299.16 ± 1.94mg / kg to 190.00 ± 7.61 mg/kg (52.86 %) at the end of 21st day indicating that blood  glucose  levels was significantly (P ≤ 0.05) decreased compared to diabetic control groups. The sub-acute toxicity study of methanol leaf extract of Aloe does not produce any significant toxicity symptoms in body weights and histological changes were observed in pancreas, liver and kidney. These data suggest that Aloe CIM- Sheetal leaf extract have ameliorative hypoglycemic effect and could be a useful source of an antidiabetic agent.

Keywords


Aloe CIM-Sheetal; fasting blood glucose; Diabetes mellitus; Streptozotocin; herbal medicine.

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References


Chandra Sekhar Singh Bhaludra, Rama Rao B, Adhrvana Chari M, Chakrapani P, Latha T, Venkatesh K , Arun Jyothi B, Amareswari P, RojaRani A. Cultivation, Phytochem. Studies, Bio. Activities and Medicinal uses of Aloe ferox Grandfather of Aloes an Important Medicinal plant. Int. Journal of Pharmacolog y 2013; vol.9 (7): 405 - 415.

Anoop A, Jegadeesan A, Subramaniam S. Toxicological study on Lingachenduram I, a siddha drug. Ind. Jrnl. Pharm. Sci 2002; vol. 64: 53-58.

Radha Mahavi YR, Swapna RS, Pradeepkiran JA, Ismail SM, Madhuri E, Bhaskar M Protective role of Ethanolic extract of Aloe vera anti-oxidant properties on Liver and kidney of streptozotozotocin-induced diabetic rats. Digest Jrnl of Nanomaterials and Biostructures 2012; vol. 7: 175-184.

Saritha V and Anilkumar K R . Toxicollogical evaluation of methanol extract of Aloe vera in rats. Int. Journal on Pharmaceutical and Biomedical Research 2010; 1 (5): 142-149.

Chandra Sekhar Singh B, Hari Y, Farhhan SC, Rama Rao B, Basha SD , Roja Rani A . Genetic Diversity analysis in the genus Aloe vera (L.) using RAPD and ISSR markers. Int. Jrnl of Pharmacology 2014; vol. 10 (8): 479 - 486.

Ramachandran A, Sneha latha C, Viswanathan V. Burden of Type 11 diabetes and its complications. The Indian Scenario. Current Sci 2002; 83: 1471-76.

Saghir A Jafri, Syed S Hasan, Aftab Nadeem, Kalsoom, Javed Iqbal. Hypoglycemic effect of Aloe vera extract in Alloxan- induced diabetic albino rats. Medicinal Journal of Islamic world Acadamy of Sciences 2011; 19 (3):127-130.

Junod A, Lambert AE, Atauffacher W, Renod AE. Diabetogenic action of streptozotocin relationship of dose to metabolic response. Jrnl.of Clin Invest 1969; 48: 2129-2139.

Herr RR, Jahnke JK, Argoudelis AD. The structure of streptozotocin. Jrnl. Am.Chem Soc 1967; 89: 4808- 4809.

Takamoto I, Kadowaki T. Nippon Rinsho 2011; 69: 563-72.

Gaber E, El Desoky, Mourad AM, Aboul-Soud and Khalid S, Al- Numair. Antidiabetic and hypolipidemic effects of Ceylon Cinnamon (Cinnamomus Verum) in alloxan-diabetic rats. Journal of Medicinal plant Research 2012; 6(9): 1685-1691.

WHO. General guide lines for methodologies on research and evaluation of traditional medicine, WHO. Geneva. 2000

The organization of Economic CO-operation and Development (OECD). The OECD guideline for testing of chemical, Acute Oral Toxicity 2001; 420.

Ogbonnia SO, Odimegwu JI, Enwuru VN. Evaluation of Hypoglycemic and Hypolipidemic effects of ethanolic extracts of Treculiaafricana Decne and Bryophyllum pinnatum Lamand their mixture on streptozotocin (STZ) - induced diabetic rats. African Journal of Biotech 2008; 7: 2535-2539.

Szkudelski T. The mechanism of alloxan and streptozotocin action in β cells of the pancreas. Physiol. Res 2001; 50: 537-546.

Bedoya FJ, Solono F, Lucas MN. Monomethyl-arginine and nicotinamide prevent streptozotocin-induced double strand DNA break formation in pancreatic rat islets. Experientia 1996; 52: 344-347.

Bolaffi JL, Nagamatsu S, Harris J, Grodsky GM. Protection by thimidine, an inhibitor of poly adenosine diphosphateribosylation, of streptozotocin inhibition of insulin secretion. Endocri 1987; 120: 2117-2122.

West E, Simon OR, Morrison EY. Streptozotocin alerts pancreatic β cell responsiveness to glucose within six hours of injection into rats. West Indian Med. Jrnl 1996; 45: 60-62.

Erah PO, Suide GEO, Omogbai EKI. Hypoglycemic effect of the extract of Solenostemonmonostachys leaves. J. West Afr. Pharma 1996; 10: 21-27.

Takasu N, Komiya I, Asawa T, Nagasawa Y, Yamada T. Streptozotocinand alloxan-induced H2O2 generation and DNA fragmentation in pancreatic islets. H2O2 as mediator for DNA fragmentation. Diabetes 1991; 40: 1141-1145.

Jafri MA, Aslam M, Kalim J, Surender S. Effect of Punicagranatum Linn.(Flowers) on blood glucose level in normal and alloxan-induced diabetic rats. Jrnl. Ethnopharmcol 2000; 70: 309 - 314.

Gillespie KM. Type I Diabetes Pathogenesis and prevention. Canadian Medical Association Journal 2006; 175: 165-170.




DOI: http://dx.doi.org/10.21746/ijbio.2015.06.0012

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