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Detection of entero toxigenicity of staphylococcus aureus isolated from community and hospital food handlers in Makkah, Saudi Arabia

Omar B. Ahmed, Anas S. Dablool


Enterotoxin production by Staphylococcus aureus has been recognized as a major health problem. Staphylococcus enterotoxins have been isolated in the community and hospitals environments. The present study detected the prevalence of S. aureus and SEs in food handlers of community- and hospital-located kitchens. About 400 adult food handlers, who working in community and hospitals kitchens were selected in the study.  S. aureus was detected in 88 and 61 of food handlers in community and hospital-located kitchens respectively. Staphylococcus enterotoxins were shown in 47.7% in food handlers of community-located kitchens and in 55.8% in those of hospital-located kitchens. Staphylococcus enterotoxin type A was the most predominant. It was concluded that the incidence of Staphylococcus aureus was higher in food handlers from community located-kitchens and there was no significant difference in the staphylococcal enterotoxigenicity between community- and hospital-located kitchens.


S. aureus; Enterotoxins; Food handlers; Community; Hospitals; Hand swabs; Nasal swabs.

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Soares MJ, Tokumaru-Miyazaki NH, Noleto AL, Figueiredo AM. Enterotoxin production by Staphylococcus aureus clones and detection of Brazilian epidemic MRSA clone (III:: B:A) among isolates from food handlers, J Med Microbiol, 1997, 46(3), 214-221.

Kadariya J, Smith TC, Thapaliya D. Staphylococcus aureus and Staphylococcal Food – Borne Disease: An Ongoing Challenge in Public Health. BioMed Research International, 2014, vol. 2014, Article ID 827965.

Kluytmans J, Van Belkum, A.; Verbrugh, H. Nasal Carriage of Staphylococcus aureus: Epidemiology, Underlying Mechanisms, and Associated Risks, Clin Microbiol Rev, 1997, 10, 505–520

Colombari V, Mayer MD, Laicini ZM, Mamizuka E, Franco BD, Destro MT, Landgrave M: Foodborne outbreak caused by Staphylococcus aureus: phenotypic and genotypic characterization of strains of food and human sources, J Food Prot, 2007, 70, 489-493.

Hatakka M, Bjorkroth KJ, Asplud K, Mäki-Petäys N, Korkeala HJ. Genotypes and enterotoxicity of isolated from the hands and nasal cavities of flight-catering employees, J Food Prot, 2000, 11, 1487-1491.

Scallan E, RM Hoekstra FJ Angulo RV Tauxe MA Widdowson, SL Roy, JL Jones, PM Griffin. Foodborne illness acquired in the United States-major pathogens,” Emerging Infectious Diseases, 2011, 17(1), 7–15.

Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV: Food-related illness and death in the United States, Emerg Infect Dis, 1999, 5, 607-625.

Bass M, Ersun AS, Kivanç G. The evaluation of food hygiene knowledge, and practices of food handlers in food businesses in Turkey, Food Control, 2006, 17, 317-322.

Dablool AS, Al-Ghamdi SS. Enterotoxigenicity of Staphylococcus aureus Isolated from Food Handlers during Hajj Season in Saudi Arabia, Open Journal of Safety Science and Technology, 2011, 1, 75-78.

Wenzel RP. Healthcare workers and the incidence of nosocomial infection: can treatment of one influence the other?--a brief review, J Chemother, 1994, 6, (4), 33-37.

Heczko BP, Ho¥er U, Kasprowicz A, Pulverer G. Quantitative studies of the £ora of the nasal vestibula in relation to nasal carriage of Staphylococcus aureus, J Med Microbiol, 1981, 140, 233-241.

Daghistani HI, Issa AA, Shehabi AA. Frequency of nasal and wound isolates of Staphylococcus aureus associated with TSST-1 production in Jordanian population. FEMS Immunology and Medical Microbiology, 2000, 27(2), 95-98.

Heczko PB, M Bulanda, U Hoeffler. Nasal carriage of Staphylococcus aureus and its unfluence on hospital infections caused by meticillin-resistant strains, ZBL BAKT, 1990, 274, 333-341.

Balaban N, Van Netten P. Staphylococcus aureus and related Staphylococci in foods: Ecology, proliferation toxinogenesis, control and monitoring, J Applied Bacteriol, 1990, 19, 123s-145s.

Paul MO, A Lamikanara and DA. Aderibigbe. Nasal carriers of coagulase- positive staphylococci in Nigerien hospital community, Trans R Soc Trop Med Hyg, 1982, 76, 319- 323.

Shanson DC and DA Mc Swiggan. Operating threat acquired infection with gentamicin resistant strain of Staphylococcus aureus: Outbreaks in two hospitals attributable tone surgeon, J Hosp Infect, 1980, 1, 171-172.

Asghar A, Zafar A, Momenah A. Bacteriological and serological survey of infectious diseases among foodhadlers in Makkah, Ann Saudi Med, 2006, 26 (2), 141-144.

Ahmed OB, Mashat BH. Prevalence of classical enterotoxin genes in staphylococcus aureus isolated from food handlers in Makkah city kitchens, Asian J Science and Tech, 2014, 5, 11, 727-731

Adesiyun AA, Prabhakar P, Ali C, Lewis M. Characteristics of Staphslococcus aureus isolated from clinical and non-clinical human sources in Trinidad: susceptibility to bacteriophages and antimicrobial agents, and toxigenicity, Zentrulbl Bakteriol, 1995, 282,519-532.

Yılmaz S, Kılıç A, Karagöz A, Bedir O, Usküdar Güçlü A, Başustaoğlu AC. Investigation of various virulence factors among the hospital and community-acquired Staphylococcus aureus isolates by real-time PCR method, Mikrobiyol Bul, 2012, 46 (4), 532-45

Adesyiun AA, Raji I, Yobe V. Enterotoxigenicity of Staphylococcusu aureus from anterior nares of dining hall workers, J Food Protect 1986, 49,955-957.

Reali D. Enterotoxin A and B production in strains of Staphylococcus aureus is isolated from human beings and foods, J Hyg, 1982, 88, 103-106.



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