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Antioxidant evaluation and cytotoxic activity of Asclepias curassavica linn.

Soma Das*, Chandrashekar Naidu K., Venkateswara Rao Y.

Abstract


Asclepias curassavica L. (Aselepiadacea) has been known since ancient times for its curative properties and has been utilized for the treatment of various ailments such as tumor, asthma, fever, homeostasis, inflammation, diarrhea, and warts. In recent times a great number of chemical and pharmacological studies have been done on Asclepias curassavica L. In the present study hexane, chloroform and methanol extracts of Asclepias curassavica L. were evaluated for their antioxidant and cytotoxic activities. The in vitro antioxidant activity was conducted by determining the total phenolic content by using Folin-ciocalteu regent and DPPH free radical scavenging activity. In all the three solvent highest total phenolic content and highest DPPH free radical scavenging activity percentage was shown by chloroform extract of Asclepias curassavica L. i.e. 105.6GAEmg/gram dry material and 93.8% respectively which are nearly equal to Ascorbic acid and Gallic acid standard.  The anti-proliferative activities of Asclepias curassavica L. was also studied in vitro by using two assay i.e. ATP chemiluminescent and flow cytometry on two cell lines i.e.  HeLa and MDA-MB-231. Asclepias curassavica L. exhibited potential cytotoxic activity. Thus results indicate that this plant might be used as a potential source of natural antioxidant and as anti-cancer agent.

Keywords


Asclepias curassavica; Antioxidant activity; ATP- chemiluminescent; Flow cytometry; HeLa and MDA-MB-231 cell line.

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References


Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA 1993, 90, 7915-7922.

Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic. Res. 1995, 22, 375-383.

Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 1996, 20, 933-956.

Scalbert A, Manach C, Morand C, Remesy C, Jimenez L. Dietary polyphenols and the prevention of diseases. Crit. Rev. Food Sci. Nutr. 2005, 45, 287-306.

Hollman PC, Katan MB. Dietary flavonoids: intake, health effects and bioavailability. Food Chem. Toxicol. 1999, 37, 937-942.

Cotelle N. Role of flavonoids in oxidative stress. Curr. Top. Med. Chem. 2001, 1, 569-590.

Kupchan SM, Knox IR, Kelsey JE, Calotropinm acytoloxic principle isolated from Asclepius curassavica L Science 1964, 146:1685-1686.

Han et al., X Han, T Shen, H Lou. Dietary polyphenols and their biological significance Int. J. Mole. Sci., 8 (2007), pp. 950–988.

Singh B, Rastogi RP. Phytochemistry 1970, 9, 315-331.

Cheung HTA, Nelson CJJ. Chem. Soc., Perkin Trans. 1 1989, 1563-1570.

Malsolm SB. Chemoecology 1990, 1, 12-21.

Dussourd DE, Hoyle AM. Chemoecology 2000, 10, 11-16.

Koike K, Bevelle C, Talapatra SK, Coredell GA, Farnsworth NR. Chem. Pharm. Bull. 1980, 28, 401-405.

Kiuchi F, Fukao Y, Maruyama T, Obata T, Tanaka M, Sasaki T, Milage M, Haque MK, Tsuda Y. chem. Pharm. Bull. 1998, 46, 528-530.

Michael C Roy, Fang-Rong Chang, Hsiao-Chu Huang, Michael YN. Chiang, and Yang-Chang Wu J. Nat. Prod., 2005, 68 (10), pp 1494–1499.




DOI: http://dx.doi.org/10.21746/ijbio.2015.01.0019

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