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Isolation and characterization of heavy metal resistant cellulosimicrobium sp. From paper mill polluted soil

Dhritiman Chanda*, Sharma G. D., Jha D. K., F. Al-Otaibi

Abstract


A Gram-positive, rod-shaped, yellow-pigmented bacterium was isolated from the polluted soil of paper mill contaminated with various heavy metals. The strain was tested for its resistance to different heavy metals (Ni, Cu, Zn and Cd) by the growth in nutrient broth tubes containing various concentrations of (0.1, 0.5, 2.0, 4.0 mM). The relative growths (%) at 2mM concentration were observed as Ni (23.52%)>Zn (20.58%)>Cu (19.20%) > Cd (15.81 %). The heavy metal resistant in the isolated strain was found to be Ni>Zn>Cu>Cd at higher concentrations. The strain showed positive activity towards urease, nitrate, citrate utilization, methyl red, Malonate utilization, starch amylase and showed negative activity against ONPG, H2S production, phynylalanine, Lysine utilization, Voges Proskauer’s (VP) test, catalase and oxidase activity. The strain was found susceptible to various antibiotics Vancomycin, Streptomycin, Rifamycin Amikacin and Ciprofloxacin. In silico study was conducted to understand the major evolutionary relationship among the different strains of Cellulosimicrobium species of nucleotide sequence of 16s ribosomal RNA with the isolated strain (KC602297) of Cellulosimicrobium sp. obtained from Gene Bank.

Keywords


Heavy metal; Gram (+) positive bacteria; In silico; 16SrRNA; Gene Bank.

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Abdelatey LM, Khalil WKB, Ali TH and Mahrous KF. Heavy metal resistance and gene expression analysis of metal resistance genes in Gram-positive and Gram-negative bacteria present in Egyptian soils, J Appl Sci Env San, 2011, 6:201-211.

Abou-Shanab RAI, Berkum P and Angle JS. Heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in Ni-rich serpentine soil and in the rhizosphere of Alyssum murale, Chemosphere, 2007, 68: 360-367.

Adel Al-Gheethi AS, Noril I, Lalung J, Azan AM, Nurfarehah ZA and Kadir AB. Biosorption of heavy metals and cephalexin from secondary effluents by tolerant bacteria, Clean Technol Environ, 2014,16(1):137-148.

Altschul SF, Gish W, Miller W, Meyer EW and Lipman DJ. Basic local alignment search tool, J Mol Biol, 1990, 215: 403-410.

Bauer RW, Kirby MDK, Sherris JC and Turck M. Antibiotic susceptibility testing by standard single disc diffusion method, American Journal of Clinical Pathology, 1996, 45: 493-496.

Berg J, Tom-Petersen A and Nybroe O. Copper amendment of agricultural soil selects for bacterial antibiotic resistance in the field, Lett Appl Microbiol, 2005, 40:146-151.

Brown JM, Steigerwalt AG, Morey RE, Daneshvar MI, Romero LJ and McNeil MM. Characterization of clinical isolates previously identifiedas Oerskovia turbata: proposal of Cellulosimicrobium funkei sp. nov.and emended description of the genus Cellulosimicrobium, Int J Syst Evol Microbiol, 2006, 56:801-804.

Chatterjee S, Sau GB and Mukherjee SK. Plant growth promotion by hexavalent chromium reducing bacterial strain, Cellulosimicrobium cellulans KUCr3, World J Mirbiol Biotechnol, 2009,25:1829-1836.

Dantas G and Sommer MOA, Oluwasegun RD and Church GM. Bacteria subsisting on antibiotics, Science, 2008, 320:100-103.

Gadd GM. Metals and microorganisms: A problem definition, FEMS Microbiology Letters, 1992, 100: 197-204.

Gao Y, Miao C, Xia J, Mao L, Wang Y and Zhou P. Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial community structure, Front. Environ Sci En, 2012, 6(2):213-223.

Greg J. Universal bacterial identification by PCR and DNA sequencing of 16S rRNA Gene. In: Margret S, Theo P, Sloots GS, James CL, Halliday C and Ian WJ, Eds, PCR for Clinical Microbiology, 2010, pp.209-214.

Gupta K, Chatterjee C and Gupta B. Isolation and characterization of heavy metal tolerant Gram-positive bacteria with bio-remedial properties from municipal waste rich soil of Kestopur canal (Kolkata), West Bengal, India, Biologia, 2012, 67(5): 827-836.

Hemala L, Zhang D and Margesin R. Cold-active antibacterial and antifungal activities and antibiotic resistance of bacteria isolated from an alpine hydrocarbon-contaminated industrial site, Res Microbiol, 2014, 165(6): 447-456.

Holt JG, Sneath NR, Staley PJA and Baltimore JT. Bergey’s manual of determinative bacteriology. The Williams and Wilkins Co, USA, 1994.

Karelova E, Harichová J, Stojnev T, Pangallo D and Ferianc P. 2011. The isolation of heavy-metal resistant culturable bacteria and resistance determinants from a heavy-metal contaminated site, Biologia, 2011, 66: 18-26.

Karrash B, Parrab OH, Cidb M, Mehrensa P, Pachecob R, Urrutiab C, Valdovinosb C and Zarorb C. Effects of pulp and paper mill effluents on the microplankton and microbial self-purification capabilities of the Biobio River, Chile, Sci Total Environ, 2006,359: 194-208.

Kumar A, Bisht BS, Joshi VD and Dhewa P. Review on bioremediation of polluted environment: A management tool, Int J Environ Sci, 2011, 1:1079-1093.

Li D, Yang M, Hu J, Zhang J, Liu R, Gu X, Zhang Y and Wang Z. Antibiotic resistance profile in environmental bacteria isolated from Penicillin production wastewater treatment plant and the receiving river, Environ Microbiol, 2009, 11: 1506-1517.

Nandi S, Maurer JJ, Hofacre C and Summers AO. Gram-positive bacteria are a major reservoir of Class 1 antibiotic resistance integrons in poultry litter, Proc Natl Acad Sci USA, 2004, 101: 7118-7122.

Nasrazadani A, Tahmourespour A and Hoodaji M. Determination of bacteria resistance threshold to lead, zinc and cadmium in three industrial wastewater samples, J Environ Studies, 2011, 36:75-86.

Nies DH. Metals and their compounds in the environment. Part II. In: Anke K, Ihnat M and Stoeppler M, Eds, The Elements: Essential and Toxic Effects on Microorganisms, Weinheim, 2004.

Osborn AM, Bruce KD, Strike P and Ritchie DA. Distribution, diversity and evolution of the bacterial mercury resistance (mer) operon, FEMS Microbiol Rev, 1997, 19:239-262.

Rath CC and Subramanyam VR. Isolation of thermophilic bacteria from hot springs of Orissa, India. Geobios, 19, 25 (2-3): 113-119.

Rathnayake V N, Megharaj M, Bolan N and Naidu R. Tolerance of Heavy Metals by Gram Positive Soil Bacteria, World Academy of Science, Engineering and Technology, 2009,53: 1185-1189.

Schalk IJ, Hannauer M and Braud A. New roles for bacterial siderophores in metal transport and tolerance, Environ Microbiol, 2011, 13:2844-2854.

Schumann P, Weiss N and Stackebrandt E. Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie, 1986) as Cellulosimicrobium cellulans gen. nov., comb. Nov, Int J Syst Evol Microbiol, 2001, 5:1007-1010.

Spain A and Alm E. Implications of microbial heavy metal tolerance in the environment, Reviews in Undergraduate Research, 2003, 2: 1-6.

Sharma A, Hira P, Shakarad M and Lal R. Draft Genome Sequence of Cellulosimicrobium sp. Strain MM, Isolated from Arsenic-Rich Microbial Mats of a Himalayan Hot Spring, Genome Announc, 2014, 2(5):e01020-14.

Song JM and Wei DZ. Production and characterization of cellulases and xylanases of Cellulosimicrobium cellulans grown in pretreated and extracted bagasse and minimal nutrient medium M9, Biomass Bioenerg, 2010,34: 1930-1934.

Tamura K, Nei M and Kumar S. Products for inferring very large phylogenies by using the neighbor-joining method, Proc Natl Acad Sci USA,2004,101:11030-11035.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M and Kumar S.MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance and maximum parsimony methods, Mol Biol Evol, 2011,28:2731-2739.

Thompson JD, Higgins DG and Gibson TH.CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids, 1994, 22: 4673-4680.

Ugurlu M, Gurses A, Doger C and Yalcin M. The removal of lignin and phenol from paper mill effluents by electrocoagulation, J Environ Manag, 2007, 87: 420-428.

Wuertz S and MergeayM. The impact of heavy metals on soil microbial communities and their activities. In: van Elsas JD, Wellington EMH and Trevors JT, Eds, Modern Soil Microbiology. Marcel Decker, NY, 1997, pp.1-20.

Yoon JH, Kang SJ, Schumann P and Oh TK. Cellulosimicrobium terreum sp. nov., isolated from soil, Int J Syst Evol Microbiol, 2007, 57:2493-2497.




DOI: http://dx.doi.org/10.21746/ijbio.2015.01.008

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