Cover Image

Rapid in vitro shoot multiplication through hypocotyl segment culture in calotropis gigantea r. Br.

Amruthapriya T., Ravichandran P.*

Abstract


Micro propagation is a handy technique for rapid multiplication of plants. Micro propagation allows the production of large number of plants from small pieces of the stock plant in relatively short period of time. The seedling organs such as epicotyl and hypocotyl are very good source of starting materials for rapid regenerations. In the present study  either only 6-Benzylaminopurine (BAP) or in combination with  indole-3-acetic acid (IAA) promoted multiplication of shoots in hypocotyl and epicotyl segments at 10µM concentration. Direct regeneration of shoots from cut ends of hypocotyls is  greatly influenced by the hormonal balance and type. Only BAP could induce significant number of shoots with in a short time along with Indole-3-acetic acid  (IAA) rather than with naphthalene acetic acid (NAA) or 2,4-Dichlorophenoxyacetic acid (2,4-D).  Adventitious shoot regeneration was strongly influenced by the explant type, shoot production being highest when hypocotyls were placed on medium with 10µM BAP and in combination of IAA. Shoot elongation was also achieved on medium containing 10µM BAP and IAA. The Plant Growth Regulators (PGR) combinations like BAP and IAA were found to be very ideal growth promoting substances for inducing tissue culture regeneration in this species  through seedling organs.

Keywords


Micro propagation; Calotropis gigantea; MS medium; Plant growth regulators.

Full Text:

PDF


DOI: http://dx.doi.org/10.21746/ijbio.2014.11.0015

Refbacks

  • There are currently no refbacks.




Copyright (c) 2014 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.