Cover Image

Mangiferin quantification in some swertia species collected from south-west zone of maharashtra

Aruna Nangare*, V. D. Mendhulkar

Abstract


The present work deals with the analytical aspect of three species of Swertia viz. Swertia densifolia Griseb, Swertia minor Knobl. collected from southwest-zone of Maharashtra and Swertia lawii Syn. for quantification of mangiferin content. A xanthone derivative, mangiferin exhibits diverse pharmacological activities like anti-diabetic, anti-HIV,   anti-cancer and antioxidant properties. Quantification of xanthone compound mangiferin was done by High Performance Liquid Chromatography method using methanol: water solvent system as the mobile phase. Injection volume for species as well as standard Samples was 10µl and flow rate of 1ml/min at room temperature. Standard calibration curve was generated for quantification of Mangiferin in studied samples. Leaf extract samples were used for the analysis. The highest quantity of Mangiferin, 6.02mg/g was estimated in Swertia densifolia while Swertia minor indicated the estimate of 4.21mg/g whereas, Swertia lawii contains 0.24mg/g of mangiferin, indicating the lowest content among the three species.

Keywords


High performance liquid chromatography; Mangiferin; Swertia species.

Full Text:

PDF

References


Adams RP, Geographic variation in Juniperous silicola and J. virginiana of the southeastern United States: multivariate analyses of morphology and terpenoids, Taxon, 1986, 35, 61-76.

Adams RP, Intraspecific terpenoids variation in Juniperous scopulorum evidence for Pleistocene refugia and recolonization in western North America, Taxon, 1983, 32, 30-46.

Bagchi D, Nutraceuticals and functional foods regulations in the United States and around the world. Toxicol. 2006, 221:1-3, 513-519.

Berger MM, Shenkin, Vitamins and trace elements: Practical aspects of supplementation. Nutrition, 2006, 22, 952-955.

Bland J S, Phytonutrition, Phytotherapy and phytopharmacology, Altern. Ther. Health Med., 1996, 2, 73-76.

Brahmachari G, Mondal S, Gangopadhyay A, Gorari D, Mukhopadhyay B, Saha MS, Brahamhachari AK, Swertia (Gentianaceae): Chemical and pharmacological Aspects, J. Chem. Bio. 2004, 1, 1627-1651.

Demizu S, Oshima Y, Hiraga Y, Takahashi K, High Performance Liquid Chromatographic Analysis of Swertia herb. J. Chromatogr. 1986, 360, 307-311.

Garrido G, Gonzalez D, Lemus Y, Garcia D, Lodeiro L, Quintero G, Delporte C, Nunez-Selles AJ, Delgado R, In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG). Pharmacol. Res. 2004, 50, 143–149.

Guha S, Ghosal S, Chattopadhyay U, Antitumor, immunomodulatory and anti-HIV effect of mangiferin, a naturally occurring glucosylxanthone. J. Chemother. 1996, 42, 443–451.

Han F, Ben GY, Shi SB, A comparative study on the resistance of Kobresia humilis grown at different altitudes in Qinghai-Tibet Plateau, Acta. Ecol. Sin., 1998,18,654-659.

Hashimoto T, Yamada Y, Alkaloid biogenesis: molecular aspects, Annu. Rev. Plant Physiol. Plant Mol. Biol.,1994,45,257-285.

Ichiki H, Miura T, Kubo M, Ishihara E, Komatsu Y, Tanigawa K, Okada M, New antidiabetic compounds, mangiferin and its glucoside. J. Biol. Pharm. Bull. 1988, 21, 1389–1390.

Kirtikar KR and Basu BD. Indian medicinal plants, Allahabad, 1984, 3, 1664-1666.

Koner C, Alpine Plant Life-Functional Plant Ecology of High Mountain Ecosystems, Springer Berlin, 1999, 259-290.

Leiro J, Arranz JA, Yanez M, Ubeira FM, Sanmartin M L, Orallo F, Expression profiles of genes involved in the mouse nuclear factor kappa B signal transduction pathway are modulated by mangiferin. Int. Inmmunopharm. 2004, 4, 763–778.

Li ZY , Min ZY, Ming XX, Quan FS, Quantitative determination of Mangiferin in different parts of Swertia davidi Franch. By HPLC. Lishizhen Medicine and Materia Madica Research. DOI, 2007. 1008-1805,

Lin CN, Liou SJ, Lee TH, Chaung YC, Won SJ, Xanthone derivatives as potential anti-cancer drugs, J Pharm. Pharmacol. 1996, 48, 539-544.

Martinez G, Delgado R, Perez G, Garrido G, Nunez Selles, A. J., Sonia Leon O, Evaluation of the in-vitro antioxidant activity of Mangifera indica, Phytotherapy Res. 2000,14, 6, 424-427.

Nobel PS, Physicochemical and environmental Plant Physiology, Academic Press, San Diego, CA, 1991.

Pandey DK, Basu S, Jha TB, Screening of different east Himalayan species and populations of Swertia L. Based on exomorphology and mangiferin content, Asian pacific Journal of Tropical Biomedicine, 2012, 1450-1456.

Ramma CS, Shirode AR, Mundada AS, Kadam VJ, Nutraceuticals- an emerging era in the treatment and prevention of cardiovascular diseases. Curr. Pharm. Biotechnol, 2006, 7, 15-23.

Rastogi S, Pandey MM, Rawat AK, A new convenient method for determination of Mangiferin, an antidiabetic compound, in Mangifera indica L. J. Planar Chromat, 2007, 20, 5, 317-320.

Sanchez GM, Giuliani RL, Nunez-Selles AJ, Davison GP, Leon- Fernandez OS, Protective effects of Mangifera indica L. extract, mangiferin and selected antioxidants against TPA-induced biomolecules oxidation and peritoneal macrophage activation in mice, Pharmacol. Res., 2000, 42, 565–573.

Sato T, Kawamoto A, Tamura A, Tatsumi Y, Fujii T, Mechanism of antioxidant action of pueraria glycoside (PG)-1 (an isoflavonoid) and mangiferin (a xanthonoid). Chem. Pharm. Bull., 1992, 40, 721–724.

Selvam SP, Mangesh V, Raja V, Mahendran TS, Muthumary J, Kalaichelvan PT, Murugesan K, Antimutagenecity of Mangiferin purified from Salacia chinensis Linn. International Multidisciplinary Research Journal, 2011, 1, 1-1-5.

Suryawanshi S, Asthana RK, Gupta RC, Simultaneous estimation of mangiferin and four secoiridoids glycosides in rat plasma using liquid chromatography tandem mass spectrometry and its application to pharmacokinetic study of herbal preparation, J. Chromat. B., 2007, 858,211-219.

Talamond P, Mondolot L, Gargadennec A, Dekochko, Hamon S, Fruchier A and Campa C, First report of Mangiferin (C- Glucosyl-xanthone) isolated from leaves of wild coffea plant, Coffea pseudozanguebariea (Rubiaceae). Acta Bot. Gallica., 2008, 4, 155.

Vakil M, Mendhulkar VD, Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f) Nees, Botanical studies, 2013, 54, 49.




DOI: http://dx.doi.org/10.21746/ijbio.2014.11.0010

Refbacks

  • There are currently no refbacks.




Copyright (c) 2014 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.