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Mangiferin quantification in some swertia species collected from south-west zone of maharashtra

Aruna Nangare*, V. D. Mendhulkar


The present work deals with the analytical aspect of three species of Swertia viz. Swertia densifolia Griseb, Swertia minor Knobl. collected from southwest-zone of Maharashtra and Swertia lawii Syn. for quantification of mangiferin content. A xanthone derivative, mangiferin exhibits diverse pharmacological activities like anti-diabetic, anti-HIV,   anti-cancer and antioxidant properties. Quantification of xanthone compound mangiferin was done by High Performance Liquid Chromatography method using methanol: water solvent system as the mobile phase. Injection volume for species as well as standard Samples was 10µl and flow rate of 1ml/min at room temperature. Standard calibration curve was generated for quantification of Mangiferin in studied samples. Leaf extract samples were used for the analysis. The highest quantity of Mangiferin, 6.02mg/g was estimated in Swertia densifolia while Swertia minor indicated the estimate of 4.21mg/g whereas, Swertia lawii contains 0.24mg/g of mangiferin, indicating the lowest content among the three species.


High performance liquid chromatography; Mangiferin; Swertia species.

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