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Phytochemical screening of Datura metel linn and its antimicrobial activity of selected human pathogens

Anitha Muthusamy, Punitha v., Leena Grace Beslin*


Medicinal plants are a source of great economic value all over the world. Various medicinal plants have been used for years in daily life to treat diseases. Present study illustrates the phytochemical screening and antimicrobial activity of Datura metel. The selected plant leaves were collected and powdered. The bioactive compounds were extracted by using acetone, chloroform and water in a soxhelet extractor. The antimicrobial activity was determined by using agar disc and well diffusion method. Acetone and chloroform extracts were mixed with 1ml dimethyl sulfoxide (DMSO) and added into the well. The extract of acetone with Datura metel has shown maximum zone of inhibition against bacterial pathogens when compared to chloroform and water extract. Phytochemical analysis reflected the antimicrobial activity of Datura metel which is due to the presence of phytochemical compounds like alkaloids, terpenoids, steroids, flavonoids, saponins, phenolic compounds and tannins. The results of agar well diffusion method indicated the inhibition and it depends largely upon plant parts used and organism tested. The present study showed good inhibitory activity of acetone extracts than the chloroform and water extracts. In future studies Datura metel will be tested in the treatment of infectious diseases caused by resistant pathogenic microorganisms.


Antimicrobial activity; Datura metel; Extract; Phytochemical and Resistance.

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Agrawal P, Rai V, Singh RB, Randomized, placebo controlled single-blind trial of holy basil leaves in patients with noninsulin-dependent Diabetes mellitus. International Journal of Clinical Pharmacology and Therapeutics 1996, 34:406-409.

Akharaiyi FC, Antibacterial, Phytochemical and Antioxidant activities of Datura metel. Int. J. PharmTech Res. 2011, 3(1):478-83.

Akroum S, Satta D, Lalauoui K, Antibacterial, antioxidant, cytotoxic activities and phytochemical screening of some Algerian plants, Euro. J. Res 2009, 31(2):289-295.

Anpin Raja RD, Prakash JW, Jeeva S, Antibacterial activity of some medicinal plants used by Kani tribe, southern Western Ghats, Tamilnadu, India. In: Trivedi PC. (ed.) Ethnic tribes and medicinal plans. Jaipur: Pointer Publishers; 2010, p. 28-45.

Aqib Sayyed, Mohib Shah, Phytochemistry, pharmacological and traditional uses of Datura stramonium L. review. Journal of Pharmacognosy and Phytochemistry. 2014, 2 (5): 123-125

Arshad Hussain, Shadma Wahab, Iffat Zarin, Sarfaraj Hussain. Antibacterial activity of the leaves of Coccinia indica (W.A.) of India. Advan Biol Res. 2011, 4(5): 241-248.

Baker JT, Borris RP, Carte B, Natural product drug discovery and development: New prospective on international collaboration. Journal of Natural products. 1995, 58: 1325-1357.

Chopra RN, Nayar SL, Chopra LC, Glossary of Indian medicinal plants. Counsil of Scientific and Industrial Research, New Delhi 1986pp. 238 – 240.

Dabur R, Ali M, Singh H, Gupta J, Sharma GL, A Novel antifungal pyrrole derivative from Datura metel leaves. Pharmzie. 2004, 59:568-570.

Farombi EO, African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents. African journal of biotechnology. 2003, 2: 662-671`

Harbone JB, phytochemical methods- A guide to modern techniques of plant analysis 3rd edn. Chapman and Hall, New York, 1998, 1-150.

Irudayaraj V, Janaky M, Johnson M, Selvan N, Preliminary phytochemical and antimicrobial studies on a spike-moss Selaginella inaequalifolia (hook. & grev). Spring. Asian Pac J Trop Med. 2010, 3(12): 957-960.

Jamdhade MS, Survase SA, Kare MA, Bhuktar AS, Phytochemical studies on Datura metel linn. In Marathwada region, Maharashtra. Journal of phytology. 2010, 2(12): 46-48.

Johnson M, Wesely EG, Zahir Hussain MI, Selvan N. Invivo and in vitro phytochemical and antibacterial efficacy of Baliospermum montanum (Wïlld). Muell. Arg. Asian Pac J Trop Med. 2010, 3(11):894-897.

Koochak H, Nejad SMS, Motamedi H. Preliminary study on the antibacterial activity of some medicinal plants of Khuzestan (Iran). Asian Pac J Trop Med. 2010, 3(3): 180-184.

Manjamalai A, Sardar Sathyajith singh R, Guruvayoorappan C, Berlin Grace VM, Analysis of Phytochemical Constituents and Anti-Microbial Activity of Some Medicinal Plants in Tamilnadu, India. Global Journal of Biotechnology & Biochemistry. 2010, 5(2): 120-128.

Moghadam MS, Maleki S, Darabpour E, Motamedi H, Nejad SMS, Antibacterial activity of eight Iranian plant extracts against methicillin and cefixime restistant Staphylococcous aureus strains. Asian Pac J Trop Med. 2010, 3(4): 262-265.

Okoye TC, Akah PA, Okoli CO, Ezike AC, Mbaoji FN, Antimicrobial and antispasmodic activity of leaf extract and fractions of Stachytarpheta cayennensis. Asian Pac J Trop Med. 2010, 3(3): 189-192.

Prasanna K, Raghunathan D, Preliminary phytochemical screening and Effect of Agele marmelos extracts against Bacterial cold-water disease causing organism. World Journal of Pharmacy Research. 2014, 3(1):498-507.

Prasanna K, yuwvaranni S, preliminary phytochemical screening and antibacterial activity of datura metel and vitex negundo against bacterial cold water disease causing organism. Int J Pharm Sci.2014, 6(5): 230-233.

Pugazharasi G, Meenakshi SA, Ramesh KN, Bastin CM, Natarajan E, Screening of antimicrobial activity of Phyllanthus maderaspatensis L. J Basic Appl Biol. 2009, 3(3&4): 43-49.

Rahmatullah M, Azam MNK, Mollik MAH, Hasan MM, Hassan Al, Jahan R, Medicinal plants used by the Kavrajes of Daturatdia Ghat, Kushita district, Bangladesh. Am. Eurasian J. Sustain. Agri. 2010, 4(2): 219-29.

Siddiq AA, Ali M, Practical pharmaceutical industry. First edition, CBS Publishers and distributors, New Delhi. 1997,126-131.

Siva Sakthi S, Saranraj P, Geetha M, Antibacterial Evaluation and Phytochemical Screening of Datura metel Leaf Extracts against Bacterial Pathogens.International Journal of Pharmaceutical & Biological Archives. 2011, 2(4):1130-1136.

Suresh SN, Nagarajan N, Preliminary phytochemical and antimicrobial activity analysis of Begonia malabarica Lam. J Basic & Appl Biol. 2009,3(1&2): 59-61.

Veni T, Pushpanathan T, Investigations Of Antimicrobial And Phytochemical Analysis Of Argemone Mexicana Medicinal Plant Extracts Against Bacteria With Gastrointestinal Relevance. Asian J Pharm Clin Res. 2014, 7(2): 93-97.



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