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Phytochemical screening of Datura metel linn and its antimicrobial activity of selected human pathogens

Anitha Muthusamy, Punitha v., Leena Grace Beslin*

Abstract


Medicinal plants are a source of great economic value all over the world. Various medicinal plants have been used for years in daily life to treat diseases. Present study illustrates the phytochemical screening and antimicrobial activity of Datura metel. The selected plant leaves were collected and powdered. The bioactive compounds were extracted by using acetone, chloroform and water in a soxhelet extractor. The antimicrobial activity was determined by using agar disc and well diffusion method. Acetone and chloroform extracts were mixed with 1ml dimethyl sulfoxide (DMSO) and added into the well. The extract of acetone with Datura metel has shown maximum zone of inhibition against bacterial pathogens when compared to chloroform and water extract. Phytochemical analysis reflected the antimicrobial activity of Datura metel which is due to the presence of phytochemical compounds like alkaloids, terpenoids, steroids, flavonoids, saponins, phenolic compounds and tannins. The results of agar well diffusion method indicated the inhibition and it depends largely upon plant parts used and organism tested. The present study showed good inhibitory activity of acetone extracts than the chloroform and water extracts. In future studies Datura metel will be tested in the treatment of infectious diseases caused by resistant pathogenic microorganisms.

Keywords


Antimicrobial activity; Datura metel; Extract; Phytochemical and Resistance.

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References


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DOI: http://dx.doi.org/10.21746/ijbio.2014.11.008

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