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Fungi associated with seeds of three varieties of sorghum grown in Botswana and its control in vitro using fungicide and plant extract treatment

Khare K. B.*, Wale K, Ntshole B. G.

Abstract


Seeds of three sorghum varieties namely Segaolane, Phofu and SNK-3939 obtained from different locations in Botswana were used for detection of seed-borne fungi and their possible control by botanical and fungicides. Four hundred seeds of each variety were surface sterilized with 2% sodium hypochlorite solution before placing them on moist blotter (10 seeds/90cm Petri plate), and incubated for seven days at 22±2o C for 12 hours under continuous light and alternating with 12 hours darkness. Eight seed-borne fungi were recovered. These were Aspergillus flavus, A. niger, Alternaria alternate, A. infectoria, A. solani, Curvularia lunata, Fusarium moniliformae and F. oxysporum. Fusarium species were dominant fungi recovered from the seeds, followed by Alternaria and Aspergillus species. The fungi detected resulted in decay and rotting of seeds, and thereby reducing percentage germination of seeds (3%, 29% and 58% seed germination in Segaolane, SNK – 3939 and Phofu respectively). Seeds treated with benlate and the aqueous leaf extract of Melia azedarach effectively controlled seed-borne fungi, and enhanced seed germination to over 90% as compared to captan (75%).copper oxychloride (60%) and Dithane M 45 (50%) as compared to non-treated seeds (20%).

Keywords


Soil-borne fungi; Sorghum; seed germination; Fungicide; Melia azedarach; Botswana

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References


Agarwal UK, Seed-borne fungi and viruses of some important crops. Wainital University Press, 1981, pp 138.

Dogget H, Sorghum: Tropical Agriculture Series, Longman group limited, UK, 1988.

Gopinath A, Shekhar SH and Prakash HS, Colonization of Fusarium species in sorghum seeds and their significance, Indian Phytopathology, 1987, 40, 181 – 185.

Headrick JM and Pataky JK, Resistance to kernel infection by Fusarium moniliformae in inbred lines of sweet corn and effect of infection on emergence, 1989, 73, 889 – 892.

International Seed Testing Association, International rules for seed testing, Proceeding International Seed Test. Association, 1976, 31, 107 – 115.

Mathur SK, Mathur SB and Neergaard P, Detection of seed-borne fungi in sorghum and location of Fusarium moniliformae in the seed, Seed Sci and Technol., 1975, 3 683 – 690.

Mohanned SS and Rajasad AH, Diversity of Fusarium species on sorghum grains, Indian Phytopathology, 2004, 57, 450 – 453.

Onyike NBN and Nelson PE, Fusarium species associated with sorghum grains from Nigeria, Lesotho and Zimbabwe, Mycology, 1992, 84, 4552 -4558.

Rajni G, Seed-borne fungi in sorghum, Plant Genetic Resources Newsletter No 106, Applied Mycology Laboratory, University of Delhi, 1996

Technical Bulletin of the Ministry of Agriculture, Agric facts: Crop Husbandry, Agronomy Section, 2004

Zaman MA, Salea AKM, Rahman GMM and Islam MTT, Seed-borne fungi of mustard seeds and their control with indigenous plant extracts, Bangaladesh Journal of Plant Pathology, 1997, 13(2), 25 – 27.




DOI: http://dx.doi.org/10.21746/ijbio.2014.10.001

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