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Detection of community-acquired methicillin-resistant Staphylococcus aureus by polymerase chain reaction

Omar B. Ahmed*, Atif H. Asghar


Methicillin-resistant Staphylococcus aureus (MRSA) is considered to have emerged from S. aureus through the acquisition of staphylococcal cassette chromosome mec (SCCmec), which carries the mecA gene for methicillin resistance. Community-acquired Staphylococcus aureus (CA-MRSA) strains carry one of SCCmec types (IV or V). In addition they carry Panton-Valentine leukocidin (PVL) gene. The aim of this study was to evaluate the prevalence of CA-MRSA in Makkah hospitals by detection mecA, SCCmec types (IV and V) and PVL genes. One hundred bacterial isolates were confirmed to carry mecA gene. CA-MRSA was detected in 44% of these MRSA isolates, 30% were SCCmec type IV; 14% were SCCmec type V and 21% were harbored the PVL gene. Molecular methods are useful for diagnosis and typing of MRSA pathogens. CA-MRSA was found to be high in Makkah hospitals and hence the infection-control guidelines for of CA-MRSA are necessary to be improved.



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Brumfitt W and J, Hamilton-Miller, Methicillin-resistant Staphylococcus aureus, N Engl J Med 1989, 320, 1188–1196.

Bukharie H A, A review of community-acquired methicillin resistant Staphylococcus aureus for primary care physicians, J Family Community Med, 2010, 17(3), 117–120

El Amin N M and Faidah H S, Methicillin-resistant Staphylococcus aureus in the western region of Saudi Arabia: prevalence and antibiotic susceptibility pattern, Ann Saudi Med, 2012, 32(5), 513-516.

Takano T, Higuchi W,Otsuka T, Baranovich T, Enany S, Saito K, et al., Novel Characteristics of Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains Belonging to Multilocus SequenceType 59 in Taiwan, Antimicrob Agents Chemother, 2008, 52(3), 837-845.

CDC, Centers for Disease Control and Prevention. Four pediatric deaths from community-acquired methicillin-resistant Staphylococcus aureus-Minnesota and North Dakota, 1997–1999, MMWR Morb. Mortal, Wkly, Rep 1999, 48, 707–710.

Vandenesch F1, Naimi T, Enright MC, Lina G, Nimmo GR, Heffernan H, et al., Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwild emergence, Emerg Infect Dis, 2003, 9, 978-984.

Genestier A L, Michallet M C, Pre´vost G, Bellot G, Chalabreysse L, Peyrol S, et al., Staphylococcus aureus Panton-Valentine leukocidin directly targets mitochondria and induces Bax-independent apoptosis of human neutrophils, J Clin Investig, 2005, 115, 3117–3127.

Labandeira-Rey M F, Couzon S, Boisset E L, Brown M, Bes Y, Benito E M, et al., Staphylococcus aureus Panton-Valentine leukocidin causes necrotizing pneumonia, Science, 2007, 315:1130–1133.

Tristan A, Bes M, Meugnier H, Lina G, Bozdogan B, Courvalin P, et al., Global distribution of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus, 2006, Emerg Infect Dis, 2007, 13, 594-600.

Aires de Sousa M1 and de Lencastre H, Bridges from hospitals to the laboratory: genetic portraits of methicillin-resistant Staphylococcus aureus clones, FEMS Immunol Med Microbiol, 2004, 40, 101-111.

CLSI, National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Susceptibility Testing; Fourteenth Informational Supplement. CLSI document M100-S14, 2004 Wayne, PA, USA, Clinical and Laboratory Standards Institute.

Bollet C, Gevaudan M J, de Lamballerie X, Zandotti C and de Micco P, A simple method for the isolation of chromosomal DNA from Gram positive or acid-fast bacteria, Nucleic Acids Res 1991, 19, 1955.

Milheiriç O C, Oliveira D C and de Lencastre H. Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus, Antimicrob Agents Chemother, 2007, 51, 3374–3377.

Okuma K, Iwakawa K, Turnidge JD, Grubb WB, Bell JM, Coombs GW, et al., Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the Community, J Clin Microbiol, 2002, 40 (11), 4289-4294.

Zhang K, McClure J A, Elsayed S, Louie T and Conly J M, Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome Mec types I to V in methicillin-resistant Staphylococcus aureus, J Clin Microbiol, 2005, 43, 5026–5033.

Badiou C, Dumitrescu O, George N, Forbes A R and Drougka E, Rapid detection of Staphylococcus aureus Panton-Valentine Leukocidin in clinical specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographgic Tests, J Clin. Microbiol, 2010, 48(4), 1384-1390.

Zaman R and Dibb WL, Methicillin resistant Staphylococcus aureus (MRSA) isolated in Saudi Arabia: Epide-miology and antimicrobial resistance patterns, J Hosp Infect, 1994, 26, 297-300.

Al-Anazi A R, Prevalence of Methicillin-Resistant Staphylococcus aureus in a teaching hospital in Riyadh, Saudi Arabia, Biomedical Research, 2009, 20(1), 7-14.

Kuroda M, Ohta T, Uchiyama I, Baba T, Yuzawa H and Kobayashi I, Whole genome sequencing of meticillin-resistant Staphylococcus aureus, Lancet, 2001, 357(9264), 1225–1240.

Martineau F, Picard F J and Lansac N, Correlation between the mecAgene determined by multiplex PCR assays and the disc diffusion susceptibility patterns of Staphylococcus aureus, Chemother, 2001, 44, 231-238.

Valsesia G, Rossi M, Bertschy S and Pfyffer GE, Emergence of SCCmec type IV and SCCmec type V methicillin-resistant Staphylococcus aureus containing the Panton–Valentine leukocidin genes in a large academic teaching hospital in central Switzerland: external invaders or persisting circulators?, J Clin Microbiol, 2010, 48, 720–727.

Asghar AH, Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from tertiary care hospitals, Pak J Med Sci, 2014, 30(4), 698-702.

Chongtrakool P, Ito T, MaX X, Kondo Y, Trakulsomboon S, Tiensasitorn C et al., Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements, Antimicrob. Agents Chemother, 2006, 50, 1001-1012.

Zaraket H, Otsuka T, Saito K, Dohmae S, Takano T, Higuchi W et al., Molecular characterization of methicillin-resistant Staphylococcus aureus in hospitals in Niigata, Japan: divergence and transmission, Microbiol Immunol, 2007, 51, 171-176.

Moussa I and Shibl AM, Molecular characterization of methicillin-resistant Staphylococcus aureus recovered from outpatient clinics in Riyadh, Saudi Arabia, Saudi Med J, 2009, 30,611–617.

Krziwanek K C, Luger B, Sammer S, Stumvoll M, Stammler S, Metz-Gercek, et al., PVL-positive MRSA in Austria, Eur J Clin Microbiol Infect Dis, 2007, 26, 931-935.

Francois P S, Harbarth A, Huyge G, Renzi M, Bento A, Gervaix D, et al., Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-1995, Emerg Infect Dis, 2008, 14, 304-307.

Lee S M M, Ender R B, Adhikari J M, Smith B, Bächi B and Cook G M, Fitness cost of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus by way of continuous culture, Antimicrob Agents Chemother, 2007, 51, 1497-1499.

Popovich K J, Weinstein R A and Hota B, Are community- associated methicillin-resistant Staphylococcus aureus (MRSA) strains replacing traditional nosocomial MRSA strains?, Clin Infect Dis, 2008, 46, 787-794.



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