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In vitro antibacterial activity of ethanolic extract of pittosporum viridiflorum leaves extract against laboratory strains of selected microorganisms

Anthoney Swamy T.*, Jackie K Obey, Terer Erick Kipngetich, Miyogo Edwin


The aim of this study was to analyse the antibacterial Activity of Pittosporum viridiflorum leaves extract against laboratory strains of selected microorganisms.  Infusions of the bark of P. viridiflorum are used to treat stomach complaints, chest pain, malaria and other fever.   The mean zones of inhibition of the extract against microorganisms were 12.67±0.882 mm for Enterobacter aerogenes, 12.50±0.281mm for Escherichia coli, 11.67±0.333 mm for Proteus vulgaris, 11.67±0.000mm for Bacillus cereus and 7.67±0.333mm for Salmonella typhi.  The penicillin positive control showed large zones of inhibition and the dimethylsulfoxide negative control did not show any zone of inhibition.  This report suggests that the extract was active against all the other organisms except S. typhi because the zone of inhibitions was less than 8 mm.  Analysis of variance showed that the zones of inhibition of the extract and antibiotic control against the microorganisms were significantly different (p<0.0001). The Tukey’s honestly test further showed both significant and non-significant comparisons between the extract and controls for various bacterial organisms.  This study has shown that controlling the growth of microorganisms in vitro can be achieved by the ethanolic extract of P. viridiflorum.


Pittosporum viridiflorum; Leaves; Antibacterial; Ethanol; Extract.

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Agyare C, Dwobeng AS, Agyepong N, Duah YB, Mensah KB, Ayande PG, Adarkwa YM, Antimicrobial, Antioxidant and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC, Advances in Pharmaceutial Sciences, 2013, 692-613.

Amusan OOG, Sukati NA, Diamini PS, Sibandzze FG , Some Swazi phytomedicines and their constituents. African Journal of Biotechnology, 2007, 6, 267-272.

Anthoney ST, Obey JK, Ngule CM, In vitro Antibacterial Activity of Methanolic-Aqua Extract of Plectranthus agentatus Leaves. World Journal of Pharmaceutical Research (WJPR), 2014, 3 (1): 339-349.

Anthoney Swamy T, Omwenga Jersfrey, Analysis of Phytochemical Composition of White and Purple Sweet Potato (Ipomoea batatas [L] Lam) root. Indian Journal of Advances in Plant Research (IJAPR), 2014, Vol. 1(3), 19-22.

Anthoney ST, Jackie O, Ngule CM, In vitro control of selected pathogenic organisms by Vernonia adoensis roots. International Journal of Pharmacy and Life Sciences, 2013, 4(8): 2855-2859.

Anthoney ST, Ngule CM, Obey J, In Vitro Antibacterial activity of Methanolic-aqua extract of Tragia brevipes Leaves. Int. J. of Pharm. Life Sci, 2014, 5 (2), 3289-3294.

Anthoney ST, Ngule CM, Obey J, Preliminary phytochemical screening of methanolic-aqua extract of Acanthospermum austral leaves. International Journal of Bioassays, 2013, 2(11): 1434-1439.

Batz MB, Hoffmann S, Morris JG Jr, Ranking the risks: The 10 Pathogen-Food Combinations with the Greatest Burden on Public Health. Gainesville, FL: Emerging Pathogens Institute, University of Florida, 2011.

Brooks, Geo F, Karen CC, Janet SB, Stephen AM, Jawetz, melnick, & Adelberg’s Medical Microbiology. 24th ed. New York: McGraw Hill, 2007.

Cayzer LW, Crisp MD, Telford IRH, Aust. Syst. Bot, 2000, 13, 845–902.

Chang XL, Yaniv Z, Research and development of new drugs originating from Chinese plants, In, Yaniv, Z. & Bachrach, U. (Eds.), ‘Handbook of medicinal plants’. Pub. Food Products Press - Haworth Press, 2005, 500.

Coker C, Bakare OO, Mobley HLT, H-NS Is a repressor of the Pr. mirabilis urease transcriptional activator gene ureR. J. Bacteriol, 2000, 128 (9): 2649-2553.

De Champs C, Bonnet R, Sirot D, Chanal C, Sirot , Clinical relevance of Pr. mirabilis in hospital patients: A two year survey. J Antimicrob. Chemoth, 2000, 45: 537-539.

Edward JB, Bacillus cereus, a Volatile Human Pathogen. Clin Microbiol Rev, 2010, 23(2): 382–398.

Gabriel C, Julia N, Parul S, Katrina P, Bryant C,

Graeme E T, A report for the Rural Industries Research and Development Corporation. The Health Benefits of Traditional Chinese Plant Medicines: Weighing the scienfitic evidence, 2007, Publication No. 06/128.

Heymann DL (Ed.), Control of Communicable Diseases Manual (19th Edition). American Public Health Association, Washington, D.C, 2008, 978-0-87553-189-2,

James BK, James PN, Harry LTM, Pathogenic Escherichia Coli. Nature reviews Microbiology, 2004, 2, 123 – 140.

Janda JM, Sharon LA, The Enterobacteriaceae 2nd ed. Washington D.C.: ASM press, 2006.

Jeyachandran R, Mahesh A, Antimicrobial Evaluation of Kigelia Africana (Lam). Research Journal of Microbiolog, 2007, 2(8): 645-649.

Kokwaro JO, Medicinal plants of East Africa. University of Nairobi press, 3rd Edition, 2008, pp. 226.

Lederber, Joshua, Martin A, Encyclopedia of Microbiology. 2nd ed. San Diego, Ca: Academic Press. 2000.

Leon R, Harold E, West, Albert GB, Streptomycin in the treatment of Proteus vulgaris Meningitis. Ann Intern Med, 1950, 32(5), 960-964.

Majowicz S, Musto J, Scallan E, The global burden of nontyphoidal Salmonella gastroenteritis. Clinical Infectious Disease, 2010, 50 (6): 882-889.

Mansy MSM, Genomic fingerprinting using random amplified polymorphic DNA for discrimination between Pr. mirabilis strains. Egypt. J. Biotech, 2001, 9, 67-79.

Nataro JP, Kaper JB, A comprehensive review of the pathogenesis, epidemiology, diagnosis and clinical aspects of diarrhoeagenic E. coli. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev, 1998, 11, 142 – 201.

Ngeny LC, Magiri E, Mutai C, Mwikwabe N, Bii C, Antimicrobial Properties and Toxicity of Hagenia abyssinica (Bruce) J. F. Gmel, Fuerstia africana, T. C. E. Fries, Asparagus racemosus (Willd.) and Ekebergia capensis Sparrm. African Journal of Pharmacology and Theurapeutics, 2013, 2(3): 76-82.

Nicolau JF, Inês GM, Tiago CL, António JM, Currais, Ana Cristina F, Monya MC, Sofia A, Lima B, Pedro AG, Santos, José GB, Luis GP, Johannes, Scheffer JC, Pittosporum undulatum Vent. grown in Portugal: secretory structures, seasonal variation and enantiomeric composition of its essential oil. Flavour and Fragrance Journal, 2007, 22, 1–9.

Obey JK, Anthoney Swamy T, Ngule CM, In Vitro Control of Selected Pathogenic Organisms by Ethanolic Extract of Garcinia Kola Seeds. Int. J. Curr. Microbiol. App. Sci, 2014, 3(4), 183-196.

Obey Jackie K, Anthoney Swamy T, Antibacterial Activity of Methanolic extract of Cola nitda Seeds on Selcted Pathogenic Organism. International Journal of Current microbiology and applied sciences (IJCMAS), 2014, 3 (8), 999 -1009.

Patricia L, Cummings Frank S, Tony K, Salmonella - A Dangerous Foodborne Pathogen. Intech Open Science, 2012, chapter 1

Press JR, In Flora of Madeira, Press JR, Short MJ (eds). HMSO Publications: London, 1994, 138 –139.

Raja A, Gajalakshmi P, Mohamed MR, Drugs from the natural bio sources for hyman disease. International Journal of Pharmacology, 2010, 6, 360 – 363.

Ramanandraibe V, Rakotovao M, Andriamaharavo RN, Bessiere JM, Ravaonindrina N, Ramanoelina ARP, Composition and antimicrobial activity of the leaf and fruit essential oilof Pittosporum viridiflorum culofondis var. viridiflorum, J Essen Oil, 2000, 12(5), 650-652.

Rivera D, Obón C, J. Ethnopharmacol, 1995, 46, 73–93.

Samy J, Sugumaran M, Lee K, Herbs of Malaysia. Ed. K.M. Wong, Pub. Times Editions - Marshall Cavendish, 2005, 244 pp.

Sanders WE, Sanders CC, Enterobacter spp.: pathogens poised to flourish at the turn of the century. Clin Microbiol Rev, 1997, 10(2), 220–241.

Sankara, Neeraja M, A-Z of Microorganisms in Our Lives. Phoenix, Az.: Oryx Press, 2000.

Scallan E, Hoekstra RM, Angulo FJ, Foodborne illness acquired in the United States - major pathogens. Emerging Infectious Disesae, 2011, 17(1), 7-15.

Taye B, Giday M, Animut A, Seid J, Antimicrobial Activities of Selected Medicinal Plants in Traditional Treatment of Human Wounds In Ethiopia. Asian Pacific Journal of Trop Biomed, 2011, 1(5), 370-375.

Wilfred MO, Donald SG, Roland NN, Phytochemical studies and antioxidant activity of two South African medicinal plants traditionally used for the management of opportunistic fungal infections in HIV/AIDS patients. BMC Complementary and Alternative Medicine, 2012, 12:43, 1 -7.



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