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Nephroprotective potential of Curcuma caesia roxb. In animal model

Harikesh Maurya*, Sanjay Gandhi, Prashant Kumar, Gaurav Upadhyay, Tirath Kumar

Abstract


A perennial medicinal herb, Curcuma caesia Roxb. (Zingiberaceae) known as black turmeric in Ayurveda, used to treat human ailments such as inflammation, wounds healing and kidney disorders. The aim of present study was to evaluate the protective effect of methanolic extract of Curcuma caesia (CCE) against gentamicin (GM) induced nephrotoxicity. Nephrotoxicity was induced in rats with GM (100 mg/kg b.w.; i.p. for 8 days) and were treated with a graded dose of CCE (250 mg/kg and 500 mg/kg b.w.; p.o. for 8 days) or 0.9% sodium chloride (vehicle). Biomarkers like; plasma (urea, creatinine) and urine (urea, creatinine); renal enzymatic and non-enzymatic antioxidants along with blood profile and histopathology were evaluated in all experimental groups. GM induced significant elevation (p<0.01 and p<0.001) in physiological, haematological parameters, plasma and urine (both urea, creatinine) along with a significant reduction (p<0.01 and p<0.001) in renal enzymatic and non-enzymatic antioxidants. The graded dose of CCE treatment over GM treated rats (GM+CCE-250 and GM+CCE-500) recorded significant improvements (p<0.001 and p<0.001) in physiological, haematological parameters, plasma and urine (both urea, creatinine) along with a dose dependent significant increment (p<0.01 and p<0.001) in renal enzymatic and non-enzymatic antioxidants. The methanolic extract of Curcuma caesia Roxb. ameliorates GM induced nephrotoxicity and ability to maintain the functional baseline of the kidneys; thus validates its therapeutic use.

Keywords


Curcuma caesia Roxb. (CCE); Gentamicin (GM); Nephrotoxicity, Reduced glutathione (GSH), Oxidised glutathione (GSSG); Malonaldehyde (MDA); Catalase (CAT).

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DOI: http://dx.doi.org/10.21746/ijbio.2014.10.003

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