A simple extraction less reverse phase HPLC method for detection and quantification of cypermethrin in plasma samples

Ajay Kumar, Anu Rahal, Rajesh Mandil, Atul Prakash


This study reports a simple and rapid High Performance Liquid Chromatography (HPLC) method for estimation of the common domestic insecticide, cypermethrin in rat plasma. The method is based upon a simple step extraction of pyrethroid from plasma and assay using reverse phase C18 column as solid phase, mixture of methanol and water (87:13) as mobile phase with a flow rate of 0.9ml/min and photodiode array detection at 225nm. The limit of detection and limit of quantitation were estimated to be 0.06 and 0.1µg.ml-1, respectively. The standard curve was linear in the concentration range of 0.1-15µg.ml-1 with a correlation of 0.997. This method is advantageous for routine analysis of pyrethroids in plasma.


Cypermethrin; Extractionless; HPLC; Method; Plasma; Rat

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DOI: http://dx.doi.org/10.21746/ijbio.2013.03.002


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