Characterization and antimicrobial activity of streptomyces strains from Thai mangrove soils

Sirikan Hunadanamra, Ancharida Akaracharanya, Somboon Tanasupawat*

Abstract


Four actinomycete isolates, SAM2-1, SMP3-1, and J8-1 and J17-2 were isolated from mangrove soils collected in Samut Prakarn and Samut Songkram provinces, the inner gulf of Thailand. These isolates were identified as Streptomyces based on their phenotypic and chemotaxonomic characteristics. They contained L–diaminopimelic acid in cell wall. Their major menaquinone components were MK-9 (H6) and MK-9 (H8). On the screening of antimicrobial activity, they  could inhibit Staphylococcus aureus ATCC 6538P and Bacillus subtilis ATCC 6633 but showed weak inhibitory activity against Kocuria rhizophila ATCC 9341, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and no activity against Candida albicans ATCC 10231. Only isolate J8-1 showed strong inhibitory activity against Bacillus subtilis ATCC 6633. The isolates SAM2-1, SMP3-1, J8-1 and J17-2 were closely related to S. sundarbansensis DSM 42019T (98.4%), S. diastaticus subsp. ardesiacus JCM 5815T (97.1%), S. iranensis JCM 17327T (96.4%) and S. seoulensis NBRC 16668T (95.3%), respectively based on 16S rRNA gene analyses.

Keywords


Actinomycetes; Streptomyces; Antimicrobial Activity; Mangrove Soils

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DOI: http://dx.doi.org/10.21746/ijbio.2013.05.0016

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