Determination of antigen-specific antibody levels in fish serum by ELISA

Vasudeva Rao Y.

Abstract


Indian major carp, Labeo rohita was immunized with bovine serum albumin (BSA) and serum was collected on days 7, 14, 21 and 28 after immunization. The BSA-specific antibody levels were determined by enzyme linked immunosorbant assay (ELISA), without raising anti- fish sp. antibodies.


Keywords


Labeo rohita; Bovine serum albumin; Immunization; Antigen-specific antibody response; ELISA

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DOI: http://dx.doi.org/10.21746/ijbio.2013.05.003

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