Validated RP-HPLC method for simultaneous estimation of amlodipine besylate and clopidogrel bisulphate in bulk and tablet dosage form

Ashwini E. Patil*, Shila V. Devtalu, Nayana D. Patil, Sheetal V. Patil, Manoj M. Bari, Shashikant D. Barhate

Abstract


A simple, accurate, fast method has been developed for simultaneous estimation of Amlodipine besylate (AM) and Clopidogrel bisulphate (CL) in combined dosage form. Better resolution and results were obtained with mobile phase containing methanol: water (70:30) adjusted to pH3 with o-phosphoric acid at 238 nm by using C18 column (grace, 4.6 x 250 mm; 5 mm). Optimum retention times of 3.85 min and 7.41 min were obtained for Amlodipine besylate and Clopidogrel bisulphate respectively with the flow rate of 1.0 ml/min. Linearity range for Amlodipine besylate and Clopidogrel bisulphate was found to be in 5-25 and 150-750 mcg/ml respectively. Accuracy, Precision Repeatability study were carried and found within range.

Keywords


Amlodipine besylate (AM); Clopidogrel bisulphate (CL); RP-HPLC etc.

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DOI: http://dx.doi.org/10.21746/ijbio.2013.02.0013

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