Effect of sodium hypochlorite on shear bond strength using three different adhesive systems: an in-vitro study

Vijay Mathai*, Mano Christaine Angelo, Jayakumar K., Sarath Babu K.

Abstract


The  aim  of  this  study  was  to  evaluate  dentin  shear  bond  strength  with  and  without  sodium  hypochlorite (NaOCL)  application  using  three  adhesive  systems.  Sixty  recently  extracted  premolar  teeth  were  sectioned  at  the  cementoenamel  junction,  cleaned  and  stored in distilled water  at  room  temperature .  Teeth  were  embedded  in  acrylic  resin  blocks in  such a  way  that   buccal  dentin  surface  was  facing  up   and  the  exposed  dentin  surface  was  polished  using  a  wet  600  grit  silicon  carbide  paper.  The samples were divided in to 6 groups of 10 in each group.  Group-I - Prime  and  Bond  NT ,  Group-II - Prime  and  Bond  NT  with  5% NaOCL,  Group-III-  Single  bond,  Group-IV-  Single  bond  with  5%NaOCl, Group-V- Clearfil SE bond and  Group-VI- Clearfil SE bond with 5%NaOCl. All  the  adhesive  systems  were  applied  according  to  the  manufacturer  instructions,  except  5%NaOCl  solution  treated  for  2 min in  Group-II, Group-IV and  Group-VI.  After  curing  of  respective  adhesives  in  each  group,  brass  rings  having  4mm  internal  diameter  and  height  were  placed  over  dentin  surface.  Z-100 composite   was used to fill the ring in increments.  The  resin increments  were  light  cured  for  40s each  and  the  samples were  stored  at  370C  in  distilled  water  for  24hrs  and  submitted  for  shear  bond  strength (SBS)  testing  with  a  crosshead  speed  of  1mm/min  by  using  Universal  Testing  Machine. The data was analysed by ANOVA and Dunnet’s tests (P<0.05). There  was   significant  difference  between  groups  with  or  without  treatment  of  NaOCL  in  Prime  and  Bond  NT  groups  but  not  in  Single  Bond  and Clearfil SE Bond groups.  The  mean  average  values  of  Load  at  break(Newton) and  SBS (MPa)  in  MEAN±SD were: G-I (145.28±21.56),  (11.55±1.23),  G-II (243.29±56.42),  (19.35±4.89),  G-III  (276.27 ±13.45),  (21.98±2.18),  G-IV  (238.12±32.90),  (18.94±2.34), G-V (208.28±12.52) (16.57±1.56), G-VI (169.07±45.24), (13.45±2.19). In  this  study  it  was  found  that  the  dentin  shear  bond  strength  of  Prime  and  Bond  NT  increased  after  5%  Sodium  hypochlorite  application  while  that  of  Single Bond and Clearfil SE Bond  decreased  after  Sodium  hypochlorite  application.  However  this  being an  in-vitro  study, more  in--vivo   studies  have  to  be  carried  out  using  several  dentin  bonding  agents  to  understand  the  effectiveness of  NaOCL  on  etched  dentin  and  subsequently  its  effect  on  dentin  shear  bond  strength.

Keywords


Prime and Bond NT; Sodium hypochlorite; Shear bond strength; Dentin; Universal Testing Machine.

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DOI: http://dx.doi.org/10.21746/ijbio.2013.04.006

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