Antimutagenic effects of compounds obtained from Eclipta alba linn. against strains of Salmonella typhimurium

Prabhu Nagarajan*, Vigneshwari Ramakrishnan Sundaramoorthy, Joseph Pushpa Innocent Danialas

Abstract


In this experimental study, antimutagenic activity of compounds obtained from Eclipta alba Linn was screened by using Ames assay for detecting direct mutagenic activity and those requiring the metabolic activation. The crude compound extracted from Eclipta alba was considered as antimutagen and analyzed in this experiment. Two strains of Salmonella typhimurium, TA163 and TA96 were used to analyze the test. These two strains are confirmed as histidine requiring mutant strains. When the mutagen is added to the culture, the strain is mutated back, thereby loosing the histidine dependence for its growth. By this study, the crude compound of Eclipta alba prevents the strain to be mutated back to the non dependence for the genotyping of the Salmonella strains were performed by histidine requirement, rfa mutation analysis, UVrB mutation, R-factor analysis toxicity tests and antimutagenicity assay.  The antimutagens obtained from the plant extract were determined for antimutagenic activity against direct acting mutagens and mutagen needing activation. For direct acting mutagens, NPD (N- nitro-o-phenyl diamine), MNNG (N- methyl-N-nitro-N-nitro soguanidine) and NaNa3 (sodium azide) with 1mg of the plant extract gives 98%, 95.2% and 90.7% inhibition the reverted colonies were observed whereas the mutagen needing activation 2-AAf (2-acetyl aminofluorine) gives 96.6% inhibition was observed. These above results indicated that the extract could inhibit the mutagenicity induced by direct acting mutagens as well as mutagens needing activation. Thus the extracts isolated from the test plant have possibility of antimutagenic activity of compound and further biochemicals extracted from the test plant will be analyzed.

Keywords


Eclipta alba; Antimutagenicity; Strains of Salmonella typhimurium; NPD; MNNG; NaNa3; 2-AAf.

Full Text:

PDF

References


De Flora S, Ferguson LR, Overview of mechanisms of cancer chemo preventive agents, Mutat Res, 2005, 591, 8-15.

Zorge IS, McDonald TM, Dagnelie G, Lutein improves visual function in some patients with retinal degeneration: a pilot study via the Internet, Optometry, 2000, 71: 147-64.

De Flora S, Izooitti A, Randerath E. Bartsch H, Balansky R, Van Schooten F, Degan P, Fronza G, Walsh D, Lewats J, DNA adducts and chronic degenerative disease. Pathogenic relevance and implications in preventive medicine, Mutat Res, 1996, 366, 197-238.

Maron DM, Ames BN, Revised methods for Salmonella mutagenicity test, Mutat Res, 1983, 113, 173-215.

Silalahi J, Anticancer and health protective properties of citrus fruit components, Asia Pac J Clin Nutr, 2002, 11, 79-84.

Ames BN, Gold LS, Dietary carcinogens, environmental pollution and cancer: some misconcenptions, Med Oncol Tum Pharmacother, 1990, 7, 69-79.

Prabhu N, Joseph PI, Chinnaswamy P, Nataraja seenivasan K, Lakshmi S, In vitro evaluation of Eclipta alba against serogroups of Leptospira interrogans, Ind J Pharm Sci, 2008, 70, 788-91.

Ferguson LR, Antimutagens as cancer chemo preventive agents in the diet, Mutat Res, 1994, 307, 395-410.

Dietz A, Rudat V, Vanselow B, Rise of oxygenation in cervical lymph node metastasis during the initial course of radiochemotherapy, Otolaryngol Head Neck Surg, 1999, 85, 15-22.

Brokos B, Gasiorowski K, Szyba K, Kozubek A, Antimutagenic activity of alkylresorcinols from cereal grains, Cancer Let, 1996, 106, 109-15.

Snodderly DM, Evidence for protection against age related macular degeneration by carotenoids and antioxidant vitamins, Amer J Clin Nutr, 1995, 12, 1448-61.

Polosa K, Raghuram TC, Prasannakrishna T, Krishnaswamy K, Effect of turmeric on urinary mutagens in smokers, Mutagenesis, 1992, 7, 107-9.

Prabhu N, Suganya DG, Sowmya TS, Orange pulp extract – a novel antimutagenic compound, J Pharmacog Herb Formul, 2010, 1, 1-7.

Beukers R, Berends W, isolation and identification of the irradiation product of thymine, Biochem Biophys Acta, 1960, 41, 550-1.

Preston BD, Singer B, Loeb LA, Mutagenic potential of O4 methylthymine in vivo determined by an enzymatic approach to site specific mutagenesis, Proc Natl Acad Sci USA, 1986, 83, 8501-5.

Stadler P, Feldmann HJ, Changes in tumor oxygenation during combined treatment with split course radiotherapy and chemotherapy in patients with head and neck cancer, Radiother Oncol, 1998, 48, 157-64.

Prabhu N, Sudha EG, Annajoice P, Soumya TS, Effect of Rosa multiflora extract on chemical mutagens using Ames assay, Der Pharma Chemica, 2010, 2, 91-97.




DOI: http://dx.doi.org/10.21746/ijbio.2012.10.009

Refbacks

  • There are currently no refbacks.




Copyright (c) 2012 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.