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Treatment of rotenone induced neurodegeneration by taurine and hesperidin

Eman S. Abdel-Reheim, Basant M. Mahmoud, Hanan A. Soliman, Basma S. Ismail


Taurine (2-aminoethane sulphonic acid) is a sulphonic acid which is derived from cysteine and is widely distributed in animal tissues. It is one of the most abundant amino acid in mammals and plays several crucial roles including modulation of calcium signaling, osmoregulation and membrane stabilization. Hesperidin occurs in the cells in crystalline, feather-like aggregates or sphaerocrytalline masses and it exhibits pharmacological and biological properties such as anti-inflammatory, anticarcinogenic, inhibit bone loss, lowering of lipid, hypoglycaemic and antioxidant activities. The current study was performed to evaluate the effect of taurine and hesperidin on neurodegeneration resulted from rotenone administration by a dose of 1.5 mg/kg b.wt three times per week for two months. Also we summarize recent findings emphasizing the role of catecholamines neurotransmitters, Tyrosine hydroxylase and oxidative stress in neurodegenerative disease model. These rats received taurine and hesperidin through gastric intubation daily for one month after rotenone administration. The results revealed that taurine and hesperidin treatment significantly ameliorated the decreased levels of the catecholamines neurotransmitters and Tyrosine hydroxylase which were decreased as after rotenone injection. Moreover, taurine and hesperidin treatment ameliorated lipid peroxidation and catalase levels.


Rotenone, Taurine, Hesperidin, Neurodegeneration, Catecholamines, Oxidative Stress.

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Abdelkader NF., Safar MM. and Salem HA. Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson's Disease: Modulation of Mitochondrial Perturbations. Mol Neurobiol. (2014); 53: 810-817.

Aebi H. Catalase in vitro. Methods Enzymol. (1984); 105: 121– 126.

Amparo T., Xiaolai Z., Bjorn S. and Kerstin K. Establishment of a Survival and Toxic Cellular Model for Parkinson’s Disease from Chicken Mesencephalon. Neurotox Res. 2013; 24(2): 119–129.

Balakrishnan A. and Menon VP. Protective effect of hesperidin on nicotine induced toxicity in rats. Indian Journal of Experimental Biology. (2007); 45:194-202.

Carriere CH., Kang NH. and Nies LP. Neuroprotection by Valproic acid in an intrastriatal rotenone model of Parkinson’s disease. Neuroscience. (2014); 267: 114-121.

Chepkova AN., Sergeeva OA. and Haas HL. Long-lasting enhancement of corticostriatal transmission by taurine: role of dopamine and acetylcholine. Cell Mol Neurobiol. (2005); 25: 767-776.

Devi SL. and Anuradha CV. Oxidative and nitrosative stress in experimental rat liver fibrosis: protective effect of taurine. Environmental Toxicology and Pharmacology. (2010); 29(2): 104-110.

Eisenhofer G., Kopin IJ. and Goldstein DS. Leaky catecholamine stores: undue waste or a stress response coping mechanism? Ann. N.Y.Acad. Sci. (2004); 1018: 224-230.

Ezekiel I., Ayo JO., Tanko Y. and Magaji RA. Effect of Taurine on Acute Restraint Stress Induced Biochemical Alterations in Wistar Rats. International Journal of Scientific & Engineering Research. (2015); 6(5): 2229-5518.

Gunduluru S. and Wudayagiri R. Protective role of Bacopa Monnieri on induced Parkinson’s disease with particular reference to catecholamine system. International Journal of Pharmacy and Pharmaceutical Sciences. (2014); 6(7): 379-382.

Hiam RA., Amira AM., Eman MS. and Kamal AF. Influence of Hesperidin combined with Sinemet on genetical and biochemical abnormalities in rats suffering from Parkinson’s disease. Life Sci J. (2012); 9(4): 930-945.

Jagadeesan G. and Sankarsami PS. Hepatoprotective effects of taurine against mercury induced toxicity in rats. Journal of Environmental Biology. (2007); 28(4): 753-756.

Johnson ME. and Bobrovskaya L. An Update on The Rotenone Models of Parkinson’s Disease: Their Ability to Reproduce the Features of Clinical Disease and Model Gene–Environment Interactions. Neurotoxicology. (2015); 46: 101–116.

Kamisli S., Ciftci O., Kaya K., Cetin A., Kamisli O. and Ozcan C. Hesperidin protects brain and sciatic nerve tissues against cisplatin-induced oxidative, histological and electromyographical side effects in rats. Toxicology and Industrial Health. (2013); 31(9): 841-51.

Kuppusamy T., Jagadhesan N., Udaiyappan J., Thamilarasan M. and Mustafa ME. Antioxidant and anti-inflammatory potential of hesperidin against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-induced experimental Parkinson's disease in mice. International journal of nutrition, pharmacology, neurological diseases. (2013); 3(3): 294-302.

Kuppusamy T., Nady B., Thamilarasan M., Musthafa ME., Nagarajan RP., Subburayan K., Arokyasamy JT., Subash S. and Gilles JG. Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson's Disease. Oxid Med Cell Longev. (2013); 2013: 102741.

Lamberto R., Mohamed NM., Silvia M., Olga SL., Gregorio MS. and Frank H. Ozone Therapy: Clinical and Basic Evidence of Its Therapeutic Potential. ARCMED. (2007); D-07-00225.

Małgorzata N. and Andrzej G. The Role of the Reactive Oxygen Species and Oxidative Stress in the Pathomechanism of the Age-Related Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults. Oxidative Medicine and Cellular Longevity. (2016); 2016: 3164734.

Neha S. and Pallavi B. Effect of Cynodon dactylon on rotenone induced Parkinson’s disease. Orient Pharm Exp Med. (2012); 12(3): 167-175.

Nidhika S., Sumit J. and Puneet K. Beneficial effect of antidepressants against rotenone induced parkinsonism like symptoms in rats. Pathophysiology. (2016); 23(2): 123-34.

Nistico R., Mehdawy B., Piccirilli S. and Mercuri N. Paraquat- and rotenone-induced models of Parkinson’s disease. Int.J. Immunopathol. Pharmacol. (2011); 24(2): 313-322.

Nuramatjan A., Deyong L., Rutong R., Yilixiati X., Xiang M., Xin Z., Yi S., Hui L., Jiamin X., Yingcong M., Xianrong Q., Min Y., Feng X., Hongbin H. and Xiaoping P. Neuroprotective Effects of a Standardized Flavonoid Extract from Safflower against a Rotenone-Induced Rat Model of Parkinson’s Disease. Molecules. (2016); 21(9): 1107.

Ohkawa H., Ohishi W. and Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. (1979); 95: 351-8.

Omar ME., Eman RY., Yasser AK., Nadia AM., Rehab FA., Enayat AO. and Amany AS. The effect of cannabis on oxidative stress and neurodegeneration induced by intrastriatal rotenone injection in rats. Comparative Clinical Pathology. (2015); 24(2): 359-378.

Parhiz H., Roohbakhsh A., Soltani F., Rezaee R. and Iranshahi M. Antioxidant and anti-inflammatory properties of the Citrus flavonoids hesperidin and hesperetin: An updated review of their molecular mechanisms and experimental models. Phytother Res. (2015); 29: 323–331.

Priya N. and Vijayalakshmi K. Anti-Parkinson effect of hesperidin in combinatin with L-DOPA on 6-OHDA induced Parkinsonism in wistar rats: a neurochemical, histopathological and immunohistochemical analysis. International Journal of Pharm Tech Research. (2016); 9(4): 266-273.

Priya N., Vijayalakshmi K. and Khadira S. Investigation on the neuroprotective effects of hesperidin on behavioural activities in 6-OHDA induced parkinson model. Int J Pharm Bio Sci. (2014); 5(4): (B) 570 - 577.

Roman F. and Olaf M. Interrelation of Oxidative Stress and Inflammation in Neurodegenerative Disease: Role of TNF. Oxidative Medicine and Cellular Longevity. (2015); 2015: 610813.

Rosemberg DB., da Rocha RF., Rico EP., Zanotto-Filho A., Dias RD., Bogo MR., Bonan CD., Moreira JC., Klamt F. and Souza DO. Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain. Neuroscience. (2010); 171(3): 683–692.

Sabrina H., Alexius F., Bettina L., Ludwig S., Gabriele S. and Heidrun E. Mechanistic Investigations of the Mitochondrial Complex I Inhibitor Rotenone in the Context of Pharmacological and Safety Evaluation. Scientific Reports. (2017); 7: 45465.

Santa C., Nadia F., Giovanni EL., Elvira V., Sebastiano G., Gioacchino C. and Michele N. Neurodegenerative Diseases: Might Citrus Flavonoids Play a Protective Role? Molecules. (2016); 21(10): 1312.

Sushruta K., Hemant K., In SK. and Dong-Kug C. Reactive oxygen species and inhibitors of inflammatory enzymes, NADPH oxidase and iNOS in experimental models of Parkinson’s disease. Mediators of Inflammation. (2012); 2012: 823902.

Syed SR., Mohd MK., Ajmal A., Mohammad A., Gulrana K., Rizwana T., Hayate J., Mohammad SS. and Mohammed MS. Hesperidin ameliorates functional and histological outcome and reduces neuroinflammation in experimental stroke. Brain Research. (2011); 1420: 93–105.

Vaibhav G., Aditi A. and Anil K. Possible nitric oxide mechanism in the protective effect of hesperidin against ischemic reperfusion cerebral injury in rats. Indian J Exp Biol. (2011); 49: 609-618.

Walaa GH., Howida SA. and Susan A. Protective Effects of Ruitn and / or Hesperidin Against Doxorubicin-Induced Hepatotoxicity. International Journal of Clinical Nutrition. (2014); 2 (1): 11-17.

Wu JY. and Prentice H. Role of taurine in the central nervous system. J. Biomed. Sci. (2010); 17: S1.

Xiaohui L., Fengyuan P. and Yachen L. Protective Effect of Taurine on the Decreased Biogenic Amine Neurotransmitter Levels in the Brain of Mice Exposed to Arsenic. Adv Exp Med Biol. (2013); 776: 277-87.

Yasser AK., Iman MM., Haitham SM., Neveen AN. and Heba SA. A study on the possible therapeutic role of Panax ginseng extract against a rat model of Parkinson’s disease induced by intrastriatal rotenone injection. Int. J. Clin. Exp. Med. (2016); 9(2): 3831-3841.

Yong R., Wenhua L., Houbo J., Qian J. and Jian F. Selective Vulnerability of Dopaminergic Neurons to Microtubule Depolymerization. The journal of biological chemistry. (2005); 280(40): 34105–12.

Zhaoqiang Z., Xixun D., Huamin X., Junxia X. and Hong J. Lesion of medullary catecholaminergic neurons is associated with cardiovascular dysfunction in rotenone-induced Parkinson’s disease rats. European Journal of Neuroscience. (2015); 42(6): 2346–2355.

Zuhal Y. and Nedret K. Effects of Taurine and Age on Cerebellum Antioxidant Status and Oxidative Stress. International Journal of Gerontology. (2011); 5(3): 166-170.



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