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GnRH-1, GnIH mRNA and Luteinizing Hormone in Domestic hens (Gallus gallus domesticus) Exposed to Different Wavelengths of light

Reddy I.J., Ashish Mishra, Mondal S.

Abstract


The objective of this study was to establish the effects of red spectrum of light (650nm, treated n=12) and normal spectrum of light (450nm control=12) on GnRH-I and GnIH mRNA expression, amplitude and frequency of luteinizing hormone (LH) and egg production from 42 to 52 weeks of age in white leghorn hens. Blood samples were collected at weekly interval from both the groups. At the 47th week of age blood samples from both the groups were collected at every 3 h for 36h to study the pulsatile secretion of LH surges. GnRH and GnIH mRNA expression pattern was studied between control and treated birds. Egg production and pause days were calculated between the two groups. LH concentration in the plasma was increased significantly (P<0.01) in hens exposed to red spectrum of light. Plasma LH concentration was higher (P<0.01) in treated birds with more number of LH surges. The amplitude and frequencies of LH were advanced in birds exposed to red spectrum of light during 36 h of sampling at 3h intervals. GnRH-I mRNA concentration was significantly (P<0.01) higher, whereas GnIH mRNA was significantly (P<0.01) lower in birds exposed to red spectrum of light compared to controls. It is hypothesized that exposure of birds to red spectrum of light enhanced (P<0.01) GnRH-I mRNA, along with LH required for ovulation and egg lay. During 77 days (42-52 weeks of age) of the experimental period, egg production was increased (p<0.01) with lower incidence of pause days in the treated group. It is concluded that low GnIH mRNA and higher levels of GnRH-I mRNA, LH, lower number of pause days enabled the birds to lay more eggs by stimulating GnRH through red spectrum of light.

Keywords


Different wavelengths of light, GnRH-ImRNA, LH surges, GnIH, egg production, hen.

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References


Bedecarrats, G.Y, Shimizu, M and Zeini, M. ‘’Pituitary levels of a putative gonadotropin inhibiting hormone receptor mRNA fluctuate during a reproductive cycle in the chicken’’. Poultry. Science 85 (2006):120-120.

Bedecarrats, G.Y, McFarlane H, Maddineni, S.R, and Ramachandran, R. Gonadotropin-inhibitory hormone receptor signaling and its impact on reproduction in chickens. General and Comparative. Endocrinology, 163 (2009):7-11.

Bédécarrats, G.Y. ‘’Control of the reproductive axis: balancing act between stimulatory and inhibitory inputs’’. Poulttry Science 94 (2015): 810–815.

Chaiseha, Y and El Halawani, M.E. ‘’Neuroendocrinology of the female turkey reproductive cycle’’. Journal of Poultry Sci, 42 (2005): 87–100.

Chowdhury, V.S, Yamamoto K., Ubuka T, Bentley G.E, Hattori A and Tsutsui K. ‘’Melatonin Stimulates the Release of Gonadotropin-Inhibitory Hormone by the Avian Hypothalamus’’. Endocrinol, 151(2010):271-280.

Lal, P., Sharp, P.J., Dunn, I.C., and Talbot, RT. ‘’Absence of an effect of naloxone, an opioid antagonist, on luteinizing hormone release in vivo and luteinizing hormone-releasing hormone I release in vitro in intact, castrated, and food restricted cockerel’’. General and Comparative. Endocrinology 77(1990): 239–245.

Lewis, P.D and Morris, T.R. ‘’Poultry and colored light’’. World's Poult Sci, Journal, 56(2000):189-207.

Lewis, P.D, Ciacciariello, M, Ciccone, NA, Sharp, PJ, and Gous, R.M. ‘’Lighting regimens and plasma LH and FSH in broiler breeders’’. British. Journal of Poultry Science, 46 (2005): 349–353.

Mans, C and Taylor W.M. Update on neuroendocrine regulation and medical intervention of reproduction in birds. Veterinary Clinics of North America Exotic Animal Practice, 11(2008):83-105.

Mobarkey NA, Vitalal, N, Heiblum and Rozenboim I. ‘’The role of retinal and extra retinal photostimulation in reproductive activity in broiler breeder hens’’. Domestic Animal Endocrinoohy, 38(2010): 235 -243.

Nakane. Y, Ikegami. K., Ono, H, Yamamoto., NS,Yoshida S and Yoshimura, T. ‘’A mammalian neural tissue opsin (Opsin 5) is a deep brain’’. Proceedings’ of the National Academy of sciences (USA), 107(2010): 15264-15268;

Pitts, G.R, Nunemaker, C.S., and Moenter, S.M. ‘’Cycles of transcription and translation do not comprise the gonadotropin-releasing hormone pulse generator in GT1 cells’’. Endocrinology, 142 (2001):1858– 1864.

Presscott, N.B and Wathes, C.M. ‘’Poultry and coloured light’’. Worlds Poult Sci Journal, 56 (200):189-207.

Ranjhan, S.K. ‘’Animal Nutrition in tropics’’: Vikas Publishing house, (1993):3rd Edition. New Delhi. India.

Reddy I. J, David, C.G., Selvaraju, S, Ravi Kiran, G and Mondal S. Changes in GnRH and plasma LH concentration, steroid hormones, intersequence pause days and egg production in domestic hen exposed to different wavelengths of light during the later stages of production in domestic hen (Gallous domesticus). Tropical Animal Health and Production, 44. 6 (2012): 1311-1317

Reddy I.J, Ashish Mishra, Mondal, S, Vaibhav Awachat and Ravi Kiran. G.’’Endocrine changes in hens (Gallus gallus domesticus) exposed to red spectrum of light’’. Current Res in Poulty Scie. 6. 1 (2016): 13-17.

Rossmanith, W.G, Scherbaum, W.A and Lauritzen C. ‘’Gonadotrophin secretion during aging in postmenopausal women’’. Neuroendocrinol., 54(1991): 211–218.

Robinson, F.E and Etches R. J. ‘’Ovarian ‘’Steroidogenesis during follicular maturation in the Domestic-Fowl (Gallus-Domesticus)’’. Biology of Reproduction 35(1986):1096-1105.

Sharp, P.J. ‘’Photoperiodic regulation of seasonal breeding in birds’’. Annals of New York Academic Sciences, 1040 (2005):189–99.

Saldanha C.J, Silverman A.J, Silver, R. ‘’Direct innervation of GnRHneurons by encephalic photoreceptors in birds. Journal of Biological Rhythms’’. 16(2001):39–49.

Snedecor, G.W., and Cochran W.G. Statistical Methods (1994): 8th Edition. Iowa state Press, Anes, Iowa, USA.




DOI: http://dx.doi.org/10.21746/ijbio.2017.08.001

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