Comparison of extraction efficiency of various methods to extract L-DOPA from Mucuna pruriens (L.) DC.

Ragni Vora, Ambika N. Joshi*, Nitesh C. Joshi


Mucuna pruriens seeds are noted to be a natural source of L-DOPA and are also used as a substitute for the synthetic L-DOPA. In the present study; attempts are made to develop suitable method(s) for extraction of L-DOPA from the powdered seeds of Mucuna pruriens using different solvents and conditions. The Seed powder was subjected to 7 different extraction methods and Method 1 was subjected to various solvent concentrations. Some methods used de-fatting procedure, either the method was cold maceration or in high temperature. Soxhlet extraction was also used in one of the extraction methods. All the extracts were analyzed using RP-HPLC. Mobile Phase used was Water: Methanol: AcetoNitrile (100:60:40) (v/v) containing 0.2% Triethylamine, pH = 3.3 and monitored at 280 nm with variable wavelength UV detector. The extraction was best with Methanol Water mixture in a cold maceration technique and overall gives good extraction efficiency of 13.36 % L-DOPA and id the best method giving highest extraction efficiency. The De-fatting method was the 2nd best methods giving approximately 8.8% L-DOPA and Method 5 viz, heat reflux method gives 8.7% L-DOPA making it the 3rd best method. There are not many studies done for optimization of extraction technique for L-DOPA despite an extensive work is reported for isolation, identification and pharmacological activities of L-DOPA from various plant sources. Keeping this in view, present investigation was done to study the extraction efficiency of various extraction methods of L-DOPA content in seed extracts of Mucuna pruriens and compare it.


Comparison; Extraction; HPLC; L-DOPA; Mucuna pruriens.

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British Pharmacopoeia. HMSO Publication Centre, London I and II (1980):254, 535 and 781.

Dhanani, Tushar, Raghuraj Singh, Sonal Shah, Premlata Kumari and Satyanshu Kumar. "Comparison of green extraction methods with conventional extraction method for extract yield, L-DOPA concentration and antioxidant activity of Mucuna pruriens seed." Green Chemistry Letters and Reviews 8.2 (2015):43–8

Infante, M.E., Perez, A.M., Simao, M.R., Manda, F., Baquete, E.F., Fernandes, A.M., and Cliff, J.L "Outbreak of Acute Psychosis Attributed to Mucuna Pruriens." Lancet 336.8723 (1990):1129.

Kasture, Veena, Vijay.Sonar, Poonam Patil, Deepak Musmade. "Quantitative Estimation of L-Dopa from Polyhebal Formulation by using RP-HPLC." American Journal of Pharmtech Research 4.3 (2014):408–14.

Kuber, Ramya, and Santhrani Thaakur. "Herbs containing L- Dopa: An update." Ancient Science of Life 27.1 (2007):50–55.

Manini, Paola, Marco d'Ischia, and Prota Giuseppe. "An Unusual Decarboxylative Maillard Reaction between l-DOPA and d-Glucose under Biomimetic Conditions: Factors Governing Competition with Pictet−Spengler Condensation." The Journal of Organic Chemistry 66.15 (2001):5048–5053.

Manyam, Bala, Muralikrishnan Dhansekaran and Theodore Hare." Neuroprotective effects of the antiparkinson drug Mucuna pruriens." Phytotherapy Research 18.9 (2004):706–12.

Misra, Laxminarain, and Hildebert Wagner. “Extraction of bioactive principles from Mucuna pruriens seeds." Indian Journal of Biochemistry & Biophysics 44 (2007):56–60

Raina, Archana, and Renu Khatri. “Quantitative Determination of L-DOPA in Seeds of Mucuna Pruriens Germplasm by High Performance Thin Layer Chromatography." Indian Journal of Pharmacuetical Science 73.4 (2011):459–62

Rathod, Bhumika and Natvarlal Patel." Development of validated RP-HPLC method for the estimation of L-Dopa from Mucuna pruriens, its extracts and in Aphrodisiac formulation." International Journal of Pharma Sciences and Research 5.8 (2014):508–13.

Simuni, Tanya, and Howard Hurtig. "Levadopa: A Pharmacologic Miracle Four Decades Later." Parkinson's Disease: Diagnosis and Clinical Management (2008): 230. (Google eBook). Eds. Stewart A Factor and William J Weiner. Demos Medical Publishing

Singh, Raman, Pawan Saini, Satish Mathur, Gyanendra Singh, and Santosh Kumar. "Application of high performance liquid chromatography to the determination and validation of Levodopa in methanolic extract of Mucuna utilis." International Journal of Green Pharmacy 4.3 (2010):156–8.

Takashi, Hasegawa, Ishii Toshiyasu, Takahashi Kazunaga, Saijo Masaaki, Fukiwake Tomohide, Nagata Tomoko and Motoki Yuji."Quantitative Determination of L-DOPA in Dietary Supplements Containing Mucuna pruriens by High Performance Liquid Chromatography." Chiba Prefecture Wei Yan Annual Report No. 60 (2011):53–6

Taylor, Leslie. "Velvet Bean" The Healing Power of Rainforest Herbs (2005) Square One Publishers

The United State Pharmacopoeia. The US Pharmaceutical Convention Inc., Rockville XXI, 21st revision (1987):585–6.

Vachhani, Urmila, Manisha Trivedi, Amrita Bajaj, and Charmi P Shah. "A HPTLC method for quantitative estimation of L-dopa from Mucuna Pruriens in polyherbal aphrodisiac formulation." Research Journal of Pharmaceutical, Biological and Chemical Sciences 2.2 (2011):389–96



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