Development and validation of LC method for the estimation of lincomycin in pharmaceutical dosage forms

Murthy Y.L.N., Madhira V.N.S. Ramprasad, P. Mahesh, B. Dilip Kumar, Y. N. Sastry, P. Mani

Abstract


A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Lincomycin in tablet dosage form. An Inertsil ODS C-18, 5µm column having 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing methanol: Acetonitrile: 1%OPA(10:65:25v/v/v) was employed. The flow rate was 1.0ml/min and effluents were monitored at 258nm. Ornidazole was used as an internal standard. The retention time for Lincomycin and Ornidazole were 8.8min and 2.6 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found to be 0.05ppm and 0.15ppm respectively and recovery of Lincomycin from tablet formulation was found to be 101.307%. The proposed method was successfully applied for the quantitative determination of Lincomycin in tablet formulation.

Keywords


Lincomycin; HPLC; Internal standard; Linearity; Validation; 258nm.

Full Text:

PDF

References


Reynolds Martindale, JEF, The Extra Pharmacopoeia 31stEdition.

www.drugs.com

www.chemblink.com

Spízek J, Rezanka T, (February) Lincomycin, cultivation of producing strains and biosynthesis Appl Microbiol Biotechnol, 2004, 63 (5): 510–9.

Argoudelis AD, Eble TE, Fox JA, Mason DJ, Studies on the biosynthesis of lincomycin IV, The origin of methyl groups, Biochemistry, 1969, 8 (8): 3408–11.

Witz DF, Hessler EJ, Miller TL, Bioconversion of tyrosine into the propylhygric acid moity of lincomycin Biochemistry, 1971, 10 (7): 1128–33.

Brahme NM, Gonzalez JE, Mizsak S, Rolls JR, Hessler EJ, Hurley LH, Biosynthesis of the lincomycins 2 Studies using stable isotopes on the biosynthesis of methylthiolincosaminide moiety of lincomycin, A J Am Chem Soc, 1984a, 106:7878–7883.

Brahme NM, Gonzalez JE, Rolls JR, Hessler EJ, Mizsak S, Hurley LH, Biosynthesis of the lincomycins 1 Studies using stable isotopes on the biosynthesis of the propyl- and ethyl-Lhygric aci moieties of lincomycin A and B, J Am Chem Soc, 106:7873–7878.

Brahme NM, Gonzalez JE, Mizsak S, Rolls JR, Hessler EJ, Hurley LH, Biosynthesis of the lincomycins 2 Studies using stable isotopes on the biosynthesis of methylthiolincosaminide moiety of lincomycin, A J Am Chem Soc, 1984a, 106:7878–7883.

Brahme NM, Gonzalez JE, Rolls JR, Hessler EJ, Mizsak S, Hurley LH, Biosynthesis of the lincomycins 1 Studies using stable isotopes on the biosynthesis of the propyl- and ethyl-Lhygric aci moieties of lincomycin A and B, 1984b, J Am Chem Soc, 106:7873–7878

Kuo MS, Yurek DA, Coats JH, Li GP, Isolation and identification of 7, 8-didemethyl-8-hydroxy-5-deazariboflavin, an unusual cosynthetic factor in streptomycetes, from Streptomyces lincolnensis J Antibiot, 1989, 42 (3): 475–8.

Kuo MS, Yurek DA, Coats JH, Chung ST, Li GP, Isolation and identification of 3-propylidene-delta 1-pyrroline-5-carboxylic acid, a biosynthetic precursor of lincomycin, J Antibiot, 1992, 45 (11): 1773–7.

Brahme NM, Gonzalez JE, Rolls JR, Hessler EJ, Mizsak S, Hurley LH, Biosynthesis of the lincomycins 1 Studies using stable isotopes on the biosynthesis of the propyl- and ethyl-Lhygric aci moieties of lincomycin A and B, J Am Chem Soc, 1984b, 106:7873–7878.

Peschke U, Schmidt H, Zhang HZ, Piepersberg W, Molecular characterization of the lincomycin-production gene cluster of Streptomyces lincolnensis 78-11, Mol Microbiol, 1995, 16 (6): 1137–56.

Pissowotzki K, Mansouri K, Piepersberg W, Genetics of streptomycin production in Streptomyces griseus: molecular structure and putative function of genes strELMB2N, Mol Gen Genet, 1991, 231 (1): 113–23.

Patt TE, Horvath BA, Isolation and characterization of Ndemethyllincomycin methyltransferase In: Abstracts, 13th International Congress of Biochemistry, Amsterdam, The Netherlands, 1985, 25–30 1985.

Michal Douša Zdeněk Si, Michal Halama and Karel Lemr, , HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC–MS/MS confirmation, Journal of Pharmaceutical and Biomedical Analysis,Volume 40, Issue 4, 2006, 981-986.

GY Wei, JH Wei, CF Qian and GH Zhao Yaowu Fenxi Zazhi, RP-HPLC determination of lincomycin hydrochloride and ephedrine hydrochloride in Lin-Ma nasal drops, 2003, 23(4), 332-334




DOI: http://dx.doi.org/10.21746/ijbio.2012.09.005

Refbacks

  • There are currently no refbacks.




Copyright (c) 2012 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.