Inhibitory activity of Acetylcholinesterase (AChE) and Antioxidant activity of methanolic extract of Desmodium gangeticum (L.)

Navi Ranjan*, Manorma Kumari

Abstract


Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Desmodium gangeticum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 28.78±0.12 to 40.83±0.05 whereas DPPH radical scavenging percentage ranged from 24.68±0.72 to 42.22±0.67.


Keywords


Antioxidants, Acetylcholinesterase inhibition, Alzheimer’s disease, DPPH, Desmodium gangeticum L.

Full Text:

PDF

References


Sagar MK, A Upadhyay, K Kalpana & Upadhyay. Evaluation of antinociceptive and anti-inflammatory properties of Desmodium gangeticum (L.) in experimental animal models. Archives of Applied Science Researc. 2.4 (2010): 33-43.

Ma X, C Zheng, C Hu, K Rahman & L Qin. The genus Desmodium (Fabaceae)-traditional uses in Chinese medicine, phytochemistry and pharmacology. Journal of Ethnopharmacology. 138.2 (2011): 314-332.

Bhattacharjee A, SC Shashidhara & S Saha. Phytochemical and ethno-pharmacological profile of Desmodium gangeticum (L.) DC. A review. International Journal of Biomedical Research. 4.10 (2013), 507-515.

Joshi H, M Parle. Antiamnesic effects of Desmodium gangeticum in mice. Yakugaku Zasshi. 126 (2006): 795-804.

Birks J. Cholinesterase inhibitors for Alzheimer's disease. Cochrane Database Syst Rev. 1(2006):CD005593

Birks J, J Grimley Evans, V Iakovidou, M Tsolaki, FE Holt. Rivastigmine for Alzheimer's disease. Cochrane Database Syst Rev. 2 (2009):CD001191.

Prvulovic D, H Hampel, J Pantel. Galantamine for Alzheimer's disease. Expert Opin Drug Metab Toxicol. 6.3 (2010): 345-354.

Stryer L. Biochemistry. 4th ed., WH Freeman: San Francisco, CA. (1995):1017.

Howes MJR, NSL Perry, PJ Houghton. Plants with traditional uses and activities, relevant to the management of Alzheimer’s disease and other cognitive disorders. Phytother Res. 7 (2003): 1-18.

Hamid AA, OO Aiyelaagbe, LA Usman, OM Ameen, A Lawal. Antioxidants: Its medicinal and pharmacological applications. Afr J Pure ApplChem. 4.8 (2010): 142-151.

Lopez S, J Batisda, F Viladomat and C Codina. Acetylcholine inhibitory activity of some amarayllidaceae alkaloids and anrcissus extracts. Life Sci. 71 (2002): 251-2529.

Ellman GL, D Courtney, V Andres and RM Featherston. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7 (1961): 88-95.

Rhee IK, RM Van Rijin, K Ingkaninan and R Verpoorte. Screening for acetylcholinesterase inhibitors from amaryllidaceae using silica gel thin-layer chromatography in combinaison with bioactivity staining. J. Chromatogr. A, 915 (2001): 217-223.

Sreejayan N and MN Rao. Free radical scavenging activity of curcuminoids. Drug Res. 46 (1996):169-171.

Ferreira A, C Proença, MLM Serralheiro and MEM Araujo. The In vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J. Ethnopharmacol. 108.1 (2006): 31-37.

Cole GM, GP Lim, F Yang, B Teter, A Begum, Q Ma, ME Harris-White and SA Frautschy. Prevention of Alzheimer’s disease Omega-3 fatty acid and phenolic anti-oxidant interventions. Neurobiol. Aging, 26.1 (2005): S133-S136.

Koleva II, TA Van -Beek, JPH Lissen, A deGroot and LN Evstatieva. Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods. Phytochem Anal. 13 (2002): 8-17.

Nagulendran KR, S Velavan, R Mahesh and V Hazeena Begum. In vitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes. J Chem. 4.3 (2007): 440-449.

Mukharjee KP, V Kumar, JP Houghton. Screening of Indian medicinal plants for acetylcholinesterase inhibitory activity. Phytother Res. 21.12 (2007):1142-5.




DOI: http://dx.doi.org/10.21746/ijbio.2017.01.004

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.