Antibacterial Activity; Medicinal Plants; Phytochemicals; MDR Bacteria; Zone of Inhibition

Screening of local medicinal plant extracts against multi drugs resistance bacteria

Sayed Abdullah, Muhammad Nadeem*


Multi Drugs Resistance (MDR) bacteria are mostly resistant to most of antibiotics, this leads to several severe infections and diseases. Thus the desire of new antibiotic sources are required which direct to the screening of new medicinal plants and use against MDR pathogenic bacteria. In our study, the antibacterial activity of three different plant extracts are utilized against pathogenic bacteria in-vitro to treat the infection and disease cause by pathogenic bacteria. The extracts were isolated from Mallotus philippensis, Silybum marianum and Stachys parviflora Benth in four different solvents extracts and were tested against eight pathogenic MDR bacterial strains (Brucella abortus, Escherichia coli, Enterobacter sakazakii, Proteus vulgaris, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosa (gram negative) and Staphylococcus aureus (gram positive) through well diffusion and disc diffusion. It was found that the extracts of selected plants showed maximum activity against all bacterial strains. The recorded zones of inhibition were 8.0-26.33mm for methanolic, 6.0-17.66mm for chloroform extracts and 8.01-2.33mm for ethyl acetate extracts.  So, it is cleared from the results that the tested plant extracts have great potential as antibacterial compounds against bacteria. However, further research is required to isolate and identify the active ingredients are vital for further pharmacological evaluation. Also screening of these plants for Anticancer and Anti-diabetic activity will be significant. 


Antibacterial Activity, Medicinal Plants, Phytochemicals, MDR Bacteria, Zone of Inhibition.

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Adwan, G. and M. Mhanna (2008). "Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens." Middle-East Journal of Scientific Research 3(3): 134-139.

Ahmad, V. U., S. Arshad, S. Bader, A. Ahmed, S. Iqbal and R. B. Tareen (2006). "New phenethyl alcohol glycosides from Stachys parviflora." Journal of Asian natural products research 8(1-2): 105-111.

Ahmad, V. U., S. Arshad, S. Bader, S. Iqbal, A. Khan, S. S. Khan, J. Hussain, R. B. Tareen and A. Ahmed (2008). "New terpenoids from Stachys parviflora Benth." Magnetic Resonance in Chemistry 46(10): 986-989.

Aibinu, I., V. Ohaegbulam, E. Adenipekun, F. Ogunsola, T. Odugbemi and B. Mee (2003). "Extended-spectrum β-lactamase enzymes in clinical isolates of Enterobacter species from Lagos, Nigeria." Journal of Clinical Microbiology 41(5): 2197-2200.

Amirghofran, Z., M. Bahmani, A. Azadmehr and K. Javidnia (2007). "Immunomodulatory and apoptotic effects of Stachys obtusicrena on proliferative lymphocytes." Medical science monitor 13(6): BR145-BR150.

Ayyanar, M. and S. Ignacimuthu (2005). "Traditional knowledge of kani tribals in Kouthalai of Tirunelveli hills, Tamil Nadu, India." Journal of ethnopharmacology 102(2): 246-255.

Bisset, N. G. (1994). Max Wichtl’s herbal drugs & phytopharmaceuticals, Boca Raton, FL, CRC Press.

Demissew, S. and E. Dange (2001). Basic and applied research on medicinal Ethiopia. Proceedings of National Workshop on Conversation and sustainable Use of Medicinal plants in Ethiopia, Addis Ababa.

Elwekeel, A., A. Elfishawy and S. AbouZid (2013). "Silymarin content in Silybum marianum fruits at different maturity stages." Journal of Medicinal Plants Research 7(23): 1665-1669.

Floyd, A. G. (1989). Rainforest trees of mainland South-eastern Australia, Inkata Press.

Gangwar, M., D. Kumar, R. Tilak, T. D. Singh, S. K. Singh, R. Goel and G. Nath (2011). "Qualitative phytochemical characterization and antibacterial evaluation of glandular hairs covering of Mallotus phillippinensis fruit extract." J Pharma Res 4: 4214-4216.

Geetha, K., S. Ramakrishna, C. Sridhar and V. Murugan (2011). "Hepatoprotective Activity of Methanolic Extract of Mallotus philippensis (Lam.) muell.-arg in Rats." Asian Journal of Chemistry 23(4): 1577.

Gupta R N et al., 2010. International Journal of Research in Ayurveda and Pharmacy 1(2), 529-553

Hassanzadeh, M., S. Emami, J. Asili and Z. T. Najaran (2011). "Review of the Essential Oil Composition of Iranian Lamiaceae+." Journal of Essential Oil Research 23(1): 35-74.

Hayashi, K., T. Nagamatsu, M. Ito, T. Hattori and Y. Suzuki (1994). "Acteoside, a Component of Stachys Sieboldii MIQ, May Be a Promising Antinephritic Agent: Effect of Acteoside on Crescentic-Type Anti-GBM Nephritis in Rats." The Japanese Journal of Pharmacology 65(2): 143-151.

Hussain, T., M. Arshad, S. Khan, H. Sattar and M. S. Qureshi (2011). "In vitro screening of methanol plant extracts for their antibacterial activity." Pak J Bot 43(1): 531-538.

Jan, S., H. Khan, M. Hamayun, A. Mehmood, N. Ahmad and I.-J. Lee "Chemical Composition, Mineral Profile and Antimicrobial Activity of Stachys parviflora and Calotropis procera."

Khanavi, M., M. Sharifzadeh, A. Hadjiakhoondi and A. Shafiee (2005). "Phytochemical investigation and anti-inflammatory activity of aerial parts of Stachys byzanthina C. Koch." Journal of ethnopharmacology 97(3): 463-468.

Kukić, J., S. Petrović and M. Niketić (2006). "Antioxidant activity of four endemic Stachys taxa." Biological and Pharmaceutical Bulletin 29(4): 725-729.

Kumar, V. P., N. S. Chauhan, H. Padh and M. Rajani (2006). "Search for antibacterial and antifungal agents from selected Indian medicinal plants." Journal of ethnopharmacology 107(2): 182-188.

Lazarević, J. S., R. M. Palić, N. S. Radulović, N. R. Ristić And G. S. Stojanović (2010). "Chemical composition and screening of the antimicrobial and antioxidative activity of extracts of Stachys species." J. Serb. Chem. Soc 75: 1347-1359.

Moreillon, P., Y. Que and M. P. Glauser (2005). "Staphylococcus aureus (including staphylococcal toxic shock)." Principles and practice of infectious diseases 6: 2321-2352.

Nostro, A., M. Germano, V. D’angelo, A. Marino and M. Cannatelli (2000). "Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity." Letters in applied microbiology 30(5): 379-384.

Okigbo, R. and E. Mmeka (2008). "Antimicrobial effects of three tropical plant extracts on Staphylococcus aureus, Escherichia coli and Candida albicans." African Journal of Traditional, Complementary and Alternative Medicines 5(3): 226-229.

Rabbani, M., S. Sajjadi and H. Zarei (2003). "Anxiolytic effects of Stachys lavandulifolia Vahl on the elevated plus-maze model of anxiety in mice." Journal of Ethnopharmacology 89(2): 271-276.

Scientific, C. o., I. R. Publications and I. Directorate (1985). The Wealth of India: a dictionary of Indian raw materials & industrial products, Publications & Information Directorate, Council of Scientific & Industrial Research.

Shah, S. M. M., F. A. Khan, S. M. H. Shah, K. A. Chishti, S. Pirzada, M. A. Khan and A. Farid (2011). "Evaluation of phytochemicals and antimicrobial activity of white and blue capitulum and whole plant of Silybum marianum." World Appl Sci J 12(8): 1139-1144.

Sidhu, M. and P. Saini (2012). "Silybum marianum: a plant of high medicinal importance—a review." World J Pharm Res 1: 72-86.

Sonboli, A., P. Salehi and S. N. Ebrahimi (2005). "Essential oil composition and antibacterial activity of the leaves of Stachys schtschegleevii from Iran." Chemistry of natural compounds 41(2): 171-174.

Sonnenbichler, J., F. Scalera, I. Sonnenbichler and R. Weyhenmeyer (1999). "Stimulatory effects of silibinin and silicristin from the milk thistle Silybum marianum on kidney cells." Journal of Pharmacology and experimental therapeutics 290(3): 1375-1383.

Spring, J. R., Richland College. Journal of Biology 2421.

Velanganni, J., D. Kadamban and A. C. Tangavelou (2011). "Phytochemical screening and antimicrobial activity of the stem of Mallotus philippensis (Lam.) Muell. Arg. Var. Philippensis (Euphorbiaceae)." International Journal of Pharmacology and Pharmaceutical Sciences 3(Suppl 2): 160-163.

Vundać, V., Ž. Maleš, M. Plazibat, P. Golja and B. Cetina-Čižmek (2005). "HPTLC determination of flavonoids and phenolic acids in some Croatian Stachys taxa." JPC-Journal of Planar Chromatography-Modern TLC 18(104): 269-273.

Vundać, V. B., A. H. Brantner and M. Plazibat (2007). "Content of polyphenolic constituents and antioxidant activity of some Stachys taxa." Food chemistry 104(3): 1277-1281.

Williams, R. (2000). "Antimicrobial resistance a global threat." Essential Drug Monitor 28(29): 1.



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