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The chemopreventive role of aged garlic extract against chemically induced tongue carcinogenesis

Hala M. Abdel-Alim*, Hassan Abdel-Dayem, Basem T. Jamal Aboms, Gamal Zuelhemma, Abdelmonein M. Osman


Tongue tumors were induced in rats using Diethylnitrosamine (DEN). Aged Garlic extract (AGE) exhibits anticarcinogenic effects. This study aimed to investigate the protective effect (AGE) against DEN induced tongue cancer in rats. Twenty-four Wister rats were divided into 4 groups. In lateral surface of the tongue; Control Groups I and II, received normal saline, while Groups III and IV were injected with 60 mg/kg DENA in a dose of 0.25ml (15mg/rat of 200g). Groups II and IV received pre-treatment with aged garlic extract 250 mg/kg orally daily for 2 weeks. Specimens for clinical and histopathological examinations were retrieved after 3 and 6 weeks. Although clinical and dysplastic histopathological changes were observed in Group 3 and 4, yet, less aggressive features of malignancy were associated with AGE. Better designed study with different doses “DEN and larger samples of animals, are needed to expand the understanding of the chemopreventive effect of AGE as dietary factor to cancer.


Aged garlic extract; tongue; Diethylnitrosamine; induced carcinogenesis; Oral cancer; cancer prevention.

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Stewart BW and P Kleihues (eds). “World cancer Report.” IARC, Lyon. Section 7, (2003): 12-41. Online []

Jemal A, F Bray, M Melissa. Center, J Ferlay, E Ward, and D Forman. “Global cancer statistics.” CA Cancer J Clin. 61. 2 (2011): 69–90. Online [].

Patel V, C Leethanankul and J Gutkind. “New approaches to the understanding of the molecular basis of oral cancer.” Crit Rev Oral Biol Med. 12 (2001): 55-63. Print.

Parkin DM, P Pisani and J Ferlay. “Estimates of the worldwide incidence of 25 major cancers in 1990.” Int J Cancer. 80 (1999): 827-841. Print.

Macfarlane GJ, L Sharp, S Porter and S Franceschi. “Trends in survival from cancers of the oral cavity and pharynx in Scotland: a clue as to why the disease is becoming more common?” Br J Cancer. 73 (1996): 805-808. Print.

Shiboski CH, S Shiboski and S Silverman. “Trends in oral cancer rates in the United States, 1973-1996.” Community Dent. Oral Epidemiol. 28 (2000): 249-256. Print.

Mackenzie J, K Ah-See, N Thakker, P Sloan, AG Maran, J Bisch and GJ Macfarlane. “Increasing incidence of oral cancer amongst young persons: what is the etiology?” Oral Oncol. 36 (2000): 387-389. Print.

Atula S, R Grenman, P Laippala, S Syrjanen. “Cancer of the tongue in patients younger than 40 years. A distinct entity?” Arch Otolaryngol Head Neck Surg. 122 (1996): 1313-9. Print.

Mognetti B, F Di Carlo and GN Berta . “Animals models in oral cancer research.” Oral Oncol. 42 (2005): 448-460. Print.

Jakszyn, P, and CA Gonzalez. "Nitrosamine and related food intake and gastric and oesophageal cancer risk: A systematic review of the epidemiological evidence". World journal of gastroenterology: WJG. 12.27 (2006): 4296–303. Print.

Tanaka T. “Chemoprevention of oral carcinogenesis.” Eur J Cancer B Oral Oncol. 31.B (1995): 3-15. Print.

Schweinsberg F, M Kouros, and K Manncke. “Comparative investigations on the organotropic carcinogenic effect of different N-nitroso compounds with rat after single and chronic treatment.” J Cancer Res Clin Oncol. 95 (1975): 123-127. Print.

U.S. Department of Health and Human Services. “N-nitrosodiethylamine. In: Survey of Compounds, Which Have Been Tested for Carcinogenic Activity.” Public Health Service Publication. 1978; No. 149, pp. 193---207 Franklin Research Center, Philadelphia. Print.

Weisburger EK, JM Ward, and CA Brown. “Dibenamine: selective protection against diethylnitrosamine-induced hepatic carcinogenesis.” Toxicol Appl Pharmacol. 28 (1974): 477—484. Print.

Horrie H, M Nemoto, and N Nishimura. “Dynamics of allelochemicals from broom (Cytisus scoparius) and their accumulation in soil.” Weed Research in Japan. 34 (1989): 47-56 (in Japanese with English summary). Print.

Al-Numair KH. “Hypocholostremic and Antioxidant Effects of Garlic (Allium Sativum L) Extracts in rats Fed high cholesterol diet.” Pakistan J of Nutrition. 8 no 2 (2009):161-166. Print.

Kasuga S, N Uda, E Kyo, M Ushijima, N Morihara, and Y Itakura. “Pharmacologic activities of aged garlic extract in comparison with other garlic preparations.” J Nutr. 131 (2001): 1080-1084. Print.

Banerjee SK, PK Mukherjee, and SK Maulik. “Garlic as an antioxidant: the good, the bad and the ugly.” Polytother. Res. 17 (2003): 97-106. Print.

Borek C. “Antioxidant health effects of aged garlic extract.” J Nutr. 131 (2001): 1010- 1015. Print.

Alkreathy H, ZA Damanhouri, N Ahmed, M Selvin, SS Ali, and AM Osman. “Aged Garlic extract protects against doxorubicin-induced cardiotoxicity in rats. “Food Chem Toxico l. 48 (2010): 951-956. Print.

Homburger F. “Chemical carcinogenesis in the Syrian golden hamster. A review.” Cancer. 23 (1969): 313-338. Print.

Eveson JW. “Animal models of intra-oral chemical carcinogenesis: a review.” J Oral Pathol. 10 (1981): 129-46. Print.

Hawkins BL and FJ Hendler . “4NQO carcinogenesis: a mouse model of oral cavity squamous cell carcinoma.” Head Neck. 16 (1994): 424-432.

Salley JJ. “Experimental carcinogenesis in the cheek pouch of the Syrian Hamster.” J Dent Res. 23 (1954): 427-38. Print.

Sun H, L Yu, H Wei and G Liu. “A novel antihepatitis drug, bicyclol, prevents liver carcinogenesis in diethylnitrosamine- initiated and phenobarbital-promoted mice tumor model.” J Biomed Biotechnol. 11.2 (2012): 67-71. Print.

Sivaramakrishnan V, PN Shilpa, VR Praveen Kumar and S Niranjali Devaraj. “Attenuation of N-nitrosodiethylamine- induced hepatocellular carcinogenesis by a novel flavonol- Morin.” Chem Biol Interact. 171 (2008): 79-88. Print.

Haradaa T, A Enomotot, T Kitazawak, K Maita, and Y Shirasu. “Oral Leukoplakia and Costochondral Hyperplasia Induced by Diethylnitrosamine in Hamsters Exposed to Cigarette Smoke with or without Dietary Vitamin C. “Vet. Pathol. 24 (1987):257-264. Print.

Witte JS, MP Longnecker, CL Bird, ER Lee, HD Frankl, and RW Haile. “Relation of vegetable, fruit and grain consumption to colorectal adenomatous polyps.” Am J Epidemiol. 144 (1996):1015–1025. Print.

Rivlin RS. “Is garlic alternative medicine?” J Nutr. 136 (2006): 713–715. Print.

WuX, F Kassie, and V Mersch-Sundermann. “Induction of apoptosis in tumor cells by naturally occurring sulfur-containing compounds.” Mutat Res. 589 (2005): 81–102. Print.

Matsuura N, Y Miyamae, K Yamane, Y Nagao, Y Hamada, N Kawaguchi, T Katsuki, K Hirata, SI Sumi, et al.. “Aged garlic extract inhibits angiogenesis and proliferation of colorectal carcinoma cells.” J Nutr. 136 (2006):842–846. Print.

Xiao D, JT Pinto, GG Gundersen, and IB Weinstein. “Effects of a series of organosulfur compounds on mitotic arrest and induction of apoptosis in colon cancer cells.” Mol Cancer Ther. 4 (2005):1388–1398. Print.

Druesne N, A Pagniez, C Mayeur, M Thomas, C Cherbuy, PH Duee, P Martel, and C Chaumontet. “Diallyl disulfide (DADS) increases histone acetylation and p21waf1/cip1 expression in human colon tumor cell lines.” Carcinogenesis. 25 (2004):1227–1236. Print.

Knowles LM, and JA Milner. “Diallyl disulfide induces ERK phosphorylation and alters gene expression profiles in human colon tumor cells.” J Nutr. 133 (2003): 2901–2906. Print.

Robert V, B Mouille, C Mayeur, M Michaud, and F Blachier. “Effects of the garlic compound diallyl disulfide on the metabolism, adherence and cell cycle of HT-29 colon carcinoma cells: evidence of sensitive and resistant sub-populations.” Carcinogenesis. 22 (2001):1155–1161. Print.

Thomson M, and M Ali. “Garlic (Allium sativum): a review of its potential use as an anti-cancer agent.” Curr Cancer Drug Targets. 3 (2003):67- 81. Print.

Katsuki T, K Hirata, H Ishikawa, N Matsuura, SI Sumi, and H Itoh. “Aged garlic extract has chemopreventative effects on 1,2-dimethylhydrazine-induced colon tumors in rats.” J Nutr. 136 (2006): 847–851. Print.

Kim JY, and O Kwon. “Garlic intake and cancer risk: an analysis using the Food and Drug Administration's evidence-based review system for the scientific evaluation of health claims.” Am J Clin Nutr. 89.1 (2009):257-264. Print.

Galeone C, C Pelucchi, F Levi, E Negri, S Franceshi, R Talamini, A Giacosa, and C La Vecchia. “Onion and Garlic use and human cancer.” Am J Clin Nutr. 84.5 (2006): 1027-32. Print.



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