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Changes in seed germination, and some physiological and ultra structural aspects of Calotropis procera seedlings under heat stress

Ali Ibrahim*, Ateeq Al-Zahrani, Zakria Baka, Hussain Almalki

Abstract


Global warming is a problematic for many plants and understanding how the plants cope with temperature stress is an important objective. In this study, two experiments were conducted; the first one explored the effect of different temperatures on Calotropis procera seed germination, and the second disclosed the physiological and ultrastructural aspects of plant seedling. A temperature rise from 25°C to 30°C increased seed germination enormously, whereas seed incubation at 35°C significantly decreased its germination. The treatment of 40°C drastically inhibited seed germination. During the experimental periods, the seeds did not germinate at all at 45°C. Seed incubation at 30°C for 5 days notably enhanced seedling growth. This effect was accompanied by accelerated reserve mobilization and enzymes activity. A mild increase in lipid peroxidation (30% increase) and electrolyte leakage (45% increase) was also observed in response to these temperatures. Additionally, the plasma membrane moved away from the cell wall and became thicker in response to this temperature. Seeds exposure to 35°C for 5 days significantly reduced seedling growth. This adverse effect was accompanied with an increase in lipid peroxidation and electrolyte leakage by 100 and 170%, respectively over the values of 25°C treatment. Furthermore, the plasma membrane was damaged and double stranded with the 35°C application. Cytoplasmic vesiculation appeared in response to 30°C and 35°C treatments. Except for total soluble sugars which was decreased, all other biochemical changes in response to 35°C were comparable with those observed at 30°C treatment.


Keywords


Cell Membrane; Cytoplasm Vesiculation; Electrolyte Leakage; Enzymes; Germination; Heat Stress; Ultrastructure.

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References


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DOI: http://dx.doi.org/10.21746/ijbio.2016.09.006

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