Cover Image

Evaluation of In-vitro cytotoxic and antioxidant activity of methanolic extracts of Ipomoea carnea and Alternanthera sessilis

Anushi Jain, Soumen Roy, Ambika Joshi*, Nitesh Joshi

Abstract


Ipomoea carnea and Alternanthera sessilis are two common weeds in India which have shown to possess several medicinal properties. The present study pertains to assess the antioxidant and cytotoxicity activity of the leaf extracts of these plants. The extraction of leaf content of the plants was carried out by soxhlet method using methanol. The in vitro cytotoxicity assay was performed in vitro on vero cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. In vitro antioxidant activity was carried out by DPPH radical scavenging method. The cytotoxicity activity for I. Carnea i.e. IC50 (50% growth inhibition) value was found to be 1mg/ml and the IC50 of A.sessilis was found to be 6.5mg/ml. The antioxidant activity of I. Carnea i.e. IC50 value was found to be 1200ug/ml whereas that of A. sessilis was found to be 400ug/ml. The cytotoxic activity was higher for Ipomoea extracts as compared to Alternanthera extracts. However, the DPPH free radical scavenging activity was higher for Alternanthera sessilis extracts as compared to Ipomoea carnea extracts. Both showed high in vitro activity, which indicates the therapeutic potential of these plants.

Keywords


Antioxidant; Cytotoxicity; DPPH radical; MTT; Weeds

Full Text:

PDF

References


Adsul, V, Khatiwora, E and Deshpande, N.R. “Evaluation of Antioxidant activity of Ipomoea carnea leaves.” J. Nat. Prod. Plant Resour. 2.5 (2012): 584-588.

Ambiga, S and Jeyaraj, M. “Evaluation of In-vitro Antioxidant Activity of Ipomoea carnea Jacq.” Int. J. Curr. Microbiol.App.Sci, 4.5 (2015): 327-338.

Chanda, S., Amrutiya, N. and Rakholiya, K. “Evaluation of Antioxidant Properties of Some Indian Vegetable and Fruit Peels by Decoction Extraction Method.” American Journal of Food Technology, 8. (2013): 173-182.

Dahake, R., Roy, S., Patil, D., Chowdhary, A., Deshmukh, R.A. “Evaluation of anti-viral activity of Jatropha curcas leaf extracts against potentially drug-resistant HIV isolates.” BMC Infectious Diseases, 12. (2012): 12.

Gaur, L.B., Singh, S.B., Gaur, S.C., Saxesena, R., Parveen, S., Kumar, S. “Facts with therapeutic significance of Ipomoea carnea.” Punarnav, 2.1 (2014):141-146.

Maroyi, A. “Use of weeds as traditional vegetables in Shurugwi District, Zimbabwe.” Journal of Ethnobiology and Ethnomedicine, 9 (2013): 60.

Mervat, M., Hanan, A. “Antioxidant Activities, Total Anthocyanins, Phenolics and Flavonoids contents of Some Sweet potato Genotypes under Stress of Different Concentrations of Sucrose and Sorbitol.” Australian Journal of Basic and Applied Sciences, 3.4 (2010): 3609-3616.

Middha, A. and Purohit S. “Determination of Free Radical Scavenging activity in Herbal Supplement: Chyawanprash.” Int. J. Drug Dev. & Res, 3.1 (2011):328-333.

Murugan S. B., Reshma, A., Deepika, R., Balamurugan, S, Sathishkumar, R. “Antioxidant capacities of Amaranthus tristis and Alternanthera sessilis: A comparative study” Journal of Medicinal Plants Research, 7.30 (2013): 2230-2235.

Njoroge, N., Bussmann, W., Gemmill, B., Newton, L. & Ngumi, W. “Utilisation of weed species as sources of traditional medicines in Central Kenya.” Lyonia, 7.2 (2004):71-87.

Patel V., Patel, P., and Kajal, S. “Antioxidant Activity of Some Selected Medicinal Plants in Western Region of India.” Advances in Biological Research, 4.1 (2010): 23-26.

Rocha, F. D., Soares, A. R., Houghton, P. J., Pereira, R. C., Kaplan, M. A. C. and Teixeira, V. L. “Potential cytotoxic activity of some Brazilian seaweeds on human melanoma cells.” Phytother. Res, 21. (2007): 170–175.

Roy, S., Pawar, S., Chowdhary, A. “Evaluation of In-vitro cytotoxic and antioxidant activity of Datura metel Linn. and Cynodon dactylon Linn. extracts.” Phcog Res, 8. (2016):123-7.

Sanger, G., Widjanark, S. B. Kusnadi, J., Berhimpon, S. (2013). “Antioxidant Activity of Methanol Extract f Sea Weeds Obtained from North Sulawesi.” Food Science and Quality Management, 19. (2013).

Singh, A., Kandasamy, T. and Odhav, B, “In-vitro propagation of Alternantherasessilis (sessile joyweed), a famine food plant.” African Journal of Biotechnology, 8.21 (2009): 5691-5695.

Stepp, J.R, Moerma, D.E. “The importance of weeds in ethnopharmacology.” J. Ethnopharmacol, 75.1 (2001):19-23.




DOI: http://dx.doi.org/10.21746/ijbio.2016.08.008

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.