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Evaluation of In-vitro cytotoxic and antioxidant activity of methanolic extracts of Ipomoea carnea and Alternanthera sessilis

Anushi Jain, Soumen Roy, Ambika Joshi*, Nitesh Joshi


Ipomoea carnea and Alternanthera sessilis are two common weeds in India which have shown to possess several medicinal properties. The present study pertains to assess the antioxidant and cytotoxicity activity of the leaf extracts of these plants. The extraction of leaf content of the plants was carried out by soxhlet method using methanol. The in vitro cytotoxicity assay was performed in vitro on vero cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. In vitro antioxidant activity was carried out by DPPH radical scavenging method. The cytotoxicity activity for I. Carnea i.e. IC50 (50% growth inhibition) value was found to be 1mg/ml and the IC50 of A.sessilis was found to be 6.5mg/ml. The antioxidant activity of I. Carnea i.e. IC50 value was found to be 1200ug/ml whereas that of A. sessilis was found to be 400ug/ml. The cytotoxic activity was higher for Ipomoea extracts as compared to Alternanthera extracts. However, the DPPH free radical scavenging activity was higher for Alternanthera sessilis extracts as compared to Ipomoea carnea extracts. Both showed high in vitro activity, which indicates the therapeutic potential of these plants.


Antioxidant; Cytotoxicity; DPPH radical; MTT; Weeds

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