Cover Image

Extraction of high quality genomic DNA from Madhuca longifolia (J. Koenig ex l.) J.F. macbr.

Rohan Vilas Gavankar*, Maya S. Chemburkar

Abstract


Madhuca longifolia commonly known as the Mahua tree is a medium to large sized deciduous tree distributed in India, Nepal and Sri Lanka. It is found growing wild and cultivated. The tree is considered as a kalpavriksha by the tribal who are forest dwellers and keenly conserve this tree. The flowers and fruits are edible and the flowers are fermented to prepare mahua drink which is an integral part of their cultural heritage. The seeds are good source of edible fats and used to extract oil. Several pharmacological activities are reported using various parts of the tree. Very little information exists on the molecular aspects of Madhuca longifolia which requires high quality DNA. Optimization of DNA extraction using CTAB and SDS buffer system was standardized. A protocol for extraction of high quality DNA from tree species which are rich in polyphenols is hereby discussed.


Keywords


Madhuca longifolia; Genomic DNA; CTAB; SDS; tree species.

Full Text:

PDF

References


Surya Shekhar Das, Swati Das (Sur) and Parthadeb Ghosh, “Optimization of DNA isolation and RAPD-PCR protocol of Acanthus volubilis wall, a rare mangrove plant from Indian Sundarban, for conservation concern”, European Journal of Experimental Biology, 3(6), (2013):33-38.

Zhanguo Xin, Jeff P. Velten, Melvin J. Oliver, and John J. Burke “High-Throughput DNA Extraction Method Suitable for PCR” BioTechniques 34, (2003) :820-826.

Weising, K, Nybom, H, Wolff, K, and W. Meyer. “DNA isolation and purification. In: DNA fingerprinting in plants and fungi,” Press. Boca Raton, Florid, (1995): 44-59.

Shepherd, M., Cross M., Stoke L.R., Scott L.J., and Jones M.E. “High-Throughput DNA Extraction from Forest Tree”. Plant Molecular Biology Reporter 20: (2002): 425a-425j.

Henry, R.J. Plant DNA extraction. In: Henry, R.J. (ed.) Plant Genotyping: the DNA fingerprinting of plants. CAB International, United Kingdom, (2001): 239-249.

Varma A, Padh H & Shrivastava N. Plant genomic DNA isolation: an art or a science. Biotechnol. J. 2: (2007): 386– 392.

Sahasrabudhe A & Deodhar M, Standardization of DNA extraction and optimization of RAPDPCR conditions in Garcinia indica, International Journal of Botany, 6: (2010): 293-298.

Murray MG & Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: (1980) :4321–4325.

Doyle, J.J. and Doyle, J.J. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: (1987): 11-15.

Rogers SO & Bendich AJ. Extraction of DNA from plant tissues. In: Gelvin SB & Schilperoort RA (eds.), pp 1–10, Plant molecular biology manual. Kluwer Academic, Boston. Speta F. 1998. Hyacinthaceae. In: Kubitzki K. (ed.), The Families and Genera of Vascular Plants 3, Springer, Berlin, (1988): 261-285.

Lodhi MA, Ye GN, Weeden NF & Reisch BI. A simple and efficient method for DNA extraction from grapevine cultivars, Vitis species and Ampelopsis. Plant. Mol. Biol. Rep. 12: 6(1994) :13-15.

J. Sambrook, D.W. Russed, Molecular Cloning. A Laboratory Mannual 3rd edition. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, Yew York, USA, 2001.

Loomis, M.D. “Overcoming problems of phenolics in the isolation of plant enzymes and organelles”. Methods in Enzymology 31: (1974):528-545.

Sharma R, Mahla HR, Mohapatra T, Bhargava SC, Sharma MM) Isolating plant genomic DNA without liquid nitrogen. Plant Mol Biol Rep 21: (2003):43-50.

Pratibha Sharma Neha Joshi and Anubhuti Sharma Isolation of genomic DNA from medicinal plants without liquid nitrogen, Indian journal of experimental biology, 48: (2010) :610-614.




DOI: http://dx.doi.org/10.21746/ijbio.2016.08.005

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 International Journal of Bioassays

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

International Journal of Bioassays is a member of the Publishers International Linking Association, Inc. (PILA), CROSSREF and CROSSMARK (USA). Digital Object Identifier (DOI) will be assigned to all its published content.